Song Jae-Jun, Cho Jae Gu, Woo Jeong-Soo, Lee Heung-Man, Hwang Soon-Jae, Chae Sung-Won
Department of Otorhinolaryngology-Head and Neck Surgery, Dongguk University International Hospital, Gyeonggi, South Korea.
Int J Pediatr Otorhinolaryngol. 2009 Jun;73(6):821-4. doi: 10.1016/j.ijporl.2009.02.017. Epub 2009 Mar 19.
The epithelial cells of the middle ear and Eustachian tube must maintain an adequate mucosal defense system against various antigenic stimuli. Since toll-like receptors (TLRs) are known to play a critical role in mucosal defense, we investigated their expression in the mucosa of the tubotympanum, nasopharynx, and oral cavity of the rat.
The expression of TLR2 and TLR4 was examined in the mucosa of the tubotympanum, nasopharynx and oral cavity of the rat using real time RT-PCR and Western blot analysis.
Transcripts for TLR2 and TLR4 were detected in the mucosa of the tubotympanum, nasopharynx, and oral cavity of the rat. The expression of TLR2 and TLR4 in the middle ear was increased more than in the other anatomical areas. Differential expression of these molecules at the protein level was confirmed by Western blot analysis.
Diverse expression of TLR2 and TLR4 in different parts of the tubotympanum and upper aerodigestive tract suggests region-specific functional modulation of the innate immune system. Differential expression of subtypes of the TLR in the normal physiology of the tubotympanum and upper aerodigestive tract also suggests that they may play a role in the pathophysiology of otitis media.
中耳和咽鼓管的上皮细胞必须维持一个足够的黏膜防御系统以抵御各种抗原刺激。由于已知Toll样受体（TLRs）在黏膜防御中起关键作用，我们研究了它们在大鼠鼓室、鼻咽和口腔黏膜中的表达。
使用实时逆转录聚合酶链反应（RT-PCR）和蛋白质印迹分析检测大鼠鼓室、鼻咽和口腔黏膜中TLR2和TLR4的表达。
在大鼠鼓室、鼻咽和口腔黏膜中检测到TLR2和TLR4的转录本。中耳中TLR2和TLR4的表达比其他解剖区域增加得更多。蛋白质印迹分析证实了这些分子在蛋白质水平上的差异表达。
鼓室和上呼吸道不同部位TLR2和TLR4的多样表达提示先天性免疫系统存在区域特异性功能调节。TLR亚型在鼓室和上呼吸道正常生理中的差异表达也提示它们可能在中耳炎的病理生理学中起作用。
