Duigou Stéphane, Knudsen Kristine G, Skovgaard Ole, Egan Elizabeth S, Løbner-Olesen Anders, Waldor Matthew K
Department of Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, 136 Harrison Ave., Boston, MA 02111, USA.
J Bacteriol. 2006 Sep;188(17):6419-24. doi: 10.1128/JB.00565-06.
Although the two Vibrio cholerae chromosomes initiate replication in a coordinated fashion, we show here that each chromosome appears to have a specific replication initiator. DnaA overproduction promoted overinitiation of chromosome I and not chromosome II. In contrast, overproduction of RctB, a protein that binds to the origin of replication of chromosome II, promoted overinitiation of chromosome II and not chromosome I.
尽管霍乱弧菌的两条染色体以协调的方式启动复制,但我们在此表明,每条染色体似乎都有一个特定的复制起始因子。过量表达DnaA会促进染色体I而非染色体II的过度起始。相反,过量表达RctB(一种与染色体II的复制起点结合的蛋白质)会促进染色体II而非染色体I的过度起始。