土拉弗朗西斯菌超氧化物歧化酶B基因（sodB）缺陷型突变体对氧化应激表现出超敏反应且毒力减弱。
Superoxide dismutase B gene (sodB)-deficient mutants of Francisella tularensis demonstrate hypersensitivity to oxidative stress and attenuated virulence.
作者信息
Bakshi Chandra Shekhar, Malik Meenakshi, Regan Kevin, Melendez J Andres, Metzger Dennis W, Pavlov Vitaly M, Sellati Timothy J
机构信息
Center for Immunology and Microbial Disease, Albany Medical College, 47 New Scotland Avenue, Albany, NY 12208-3479, USA.
出版信息
J Bacteriol. 2006 Sep;188(17):6443-8. doi: 10.1128/JB.00266-06.
Abstract
A Francisella tularensis live vaccine strain mutant (sodB(Ft)) with reduced Fe-superoxide dismutase gene expression was generated and found to exhibit decreased sodB activity and increased sensitivity to redox cycling compounds compared to wild-type bacteria. The sodB(Ft) mutant also was significantly attenuated for virulence in mice. Thus, this study has identified sodB as an important F. tularensis virulence factor.
摘要
构建了一种铁超氧化物歧化酶基因表达降低的土拉弗朗西斯菌活疫苗株突变体（sodB(Ft)），发现与野生型细菌相比，该突变体表现出超氧化物歧化酶B（sodB）活性降低以及对氧化还原循环化合物的敏感性增加。sodB(Ft)突变体在小鼠中的毒力也显著减弱。因此，本研究已确定sodB是土拉弗朗西斯菌的一个重要毒力因子。
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