Pellicciari C, Ronchetti E, Tori R, Formenti D, Manfredi Romanini M G
Dipartimento di Biologia Animale, CNR, Pavia, Italy.
Basic Appl Histochem. 1990;34(1):79-85.
A method is proposed to evaluate the amount of DNA resistant to the C-banding pretreatments (C-heterochromatic-DNA) in metaphase chromosomes. Measurements were performed by microfluorometry on propidium iodide stained metaphases of man, gorilla and mouse; in these species, C-heterochromatin exhibits significant differences of both base composition and distribution along the chromosomes. The amount of C-heterochromatic-DNA was found to be about 16%, 28% and 58% of the total DNA content (genome size) in man, gorilla and mouse, respectively. The areas of C-bands after Giemsa staining were also assessed by microdensitometry, and corresponded to about 8%, 15% and 14% of the total karyotype area of man, gorilla and mouse respectively. No direct relation thus exists between C-band areas and the amount of DNA resistant to the C-banding pretreatments. In man and gorilla, the amount of C-heterochromatic-DNA accounts for the differences observed in genome size.
本文提出了一种评估中期染色体中对C带预处理有抗性的DNA量（C-异染色质DNA）的方法。通过显微荧光测定法对人、大猩猩和小鼠的碘化丙啶染色中期染色体进行测量；在这些物种中，C-异染色质在碱基组成和沿染色体的分布上均表现出显著差异。结果发现，人、大猩猩和小鼠中C-异染色质DNA的量分别约占总DNA含量（基因组大小）的16%、28%和58%。吉姆萨染色后C带的面积也通过显微密度测定法进行评估，分别对应于人、大猩猩和小鼠总核型面积的约8%、15%和14%。因此，C带面积与对C带预处理有抗性的DNA量之间不存在直接关系。在人和大猩猩中，C-异染色质DNA的量解释了基因组大小上观察到的差异。
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