C带处理后成熟小鼠精子的细胞化学研究。
A cytochemical study of mature mouse spermatozoa after C-banding treatment.
作者信息
Bianchi P G, Manicardi G C, Bizzaro D, Sakkas D, Bianchi U
机构信息
Polyclinique de Stérilité et d'Endocrinologie Gynécologique, Université de Genève, Genève, Suisse, Italy.
出版信息
Eur J Histochem. 1993;37(2):155-9.
Heterochromatin in mature mouse spermatozoa has been investigated by using C-banding treatment followed by either i) Giemsa staining, ii) staining with DAPI, a fluorochrome specific for AT rich DNA and iii) by using quantitative DNA measurements [Acriflavine-Feulgen, DAPI and Ethidium Bromide (EB)]. We have shown that a fraction of the mature sperm chromatin is affected by C-banding treatment. The sperm chromatin treated with Ba(OH)2 and stained with EB doubled fluorescence emission values found in untreated control preparations. This experimental result clearly shows that the use of intercalating fluorochromes, such as EB, is unsuitable for quantitative evaluation of highly condensed DNAs.
通过C带处理，随后进行以下操作来研究成熟小鼠精子中的异染色质：i）吉姆萨染色，ii）用DAPI染色（DAPI是一种对富含AT的DNA具有特异性的荧光染料），以及iii）使用定量DNA测量方法[吖啶黄素-福尔根染色法、DAPI和溴化乙锭（EB）]。我们已经表明，成熟精子染色质的一部分会受到C带处理的影响。用Ba(OH)2处理并用EB染色的精子染色质，其荧光发射值是未处理对照制剂中荧光发射值的两倍。这一实验结果清楚地表明，使用诸如EB等嵌入性荧光染料不适用于对高度浓缩的DNA进行定量评估。
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