适用于预制PhastGel电泳介质的脱氧核糖核酸酶酶谱分析。
Deoxyribonuclease zymography adapted to the precast PhastGel electrophoresis media.
作者信息
Straetkvern K O, Raae A J
机构信息
Department of Biochemistry, University of Bergen, Norway.
出版信息
Electrophoresis. 1990 Apr;11(4):347-9. doi: 10.1002/elps.1150110416.
PMID:1692791
Abstract
A set-up for casting fluorescent indicator agarose gels on ultrathin polyacrylamide microelectrophoresis gel media (Pharmacia PhastGel media) is described. The zymogram system allows a rapid and sensitive detection of deoxyribonuclease in various gel media, following isoelectric focusing, native and sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
摘要
本文描述了一种在超薄聚丙烯酰胺微电泳凝胶介质（Pharmacia PhastGel介质）上浇铸荧光指示剂琼脂糖凝胶的装置。该酶谱系统能够在等电聚焦、天然和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后，快速灵敏地检测各种凝胶介质中的脱氧核糖核酸酶。
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