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Isoelectric focusing of multiple forms of DNase in thin layers of polyacrylamide gel and detection of enzymatic activity with a zymogram method following separation.

作者信息

Kim H S, Liao T H

出版信息

Anal Biochem. 1982 Jan 1;119(1):96-101. doi: 10.1016/0003-2697(82)90671-6.

DOI:10.1016/0003-2697(82)90671-6
PMID:6176141
Abstract
摘要

相似文献

1
Isoelectric focusing of multiple forms of DNase in thin layers of polyacrylamide gel and detection of enzymatic activity with a zymogram method following separation.在聚丙烯酰胺凝胶薄层中对多种形式的脱氧核糖核酸酶进行等电聚焦，并在分离后用酶谱法检测酶活性。
Anal Biochem. 1982 Jan 1;119(1):96-101. doi: 10.1016/0003-2697(82)90671-6.
2
Deoxyribonuclease zymography adapted to the precast PhastGel electrophoresis media.适用于预制PhastGel电泳介质的脱氧核糖核酸酶酶谱分析。
Electrophoresis. 1990 Apr;11(4):347-9. doi: 10.1002/elps.1150110416.
3
Detection of elastase activity with a zymogram method after isoelectric focusing in polyacrylamide gel.在聚丙烯酰胺凝胶中进行等电聚焦后，采用酶谱法检测弹性蛋白酶活性。
Anal Biochem. 1984 Aug 1;140(2):472-7. doi: 10.1016/0003-2697(84)90196-9.
4
Quantitative assay of deoxyribonuclease activity after isoelectric focusing in polyacrylamide gels: pH control and effects of enzyme diffusion.聚丙烯酰胺凝胶等电聚焦后脱氧核糖核酸酶活性的定量测定：pH 控制及酶扩散的影响
Anal Biochem. 1984 Apr;138(1):99-106. doi: 10.1016/0003-2697(84)90775-9.
5
Identification of proteins according to biological activity following separation by two-dimensional isoelectric focusing/sodium dodecyl sulfate gel electrophoresis: analysis of human exocrine pancreatic proteins.二维等电聚焦/十二烷基硫酸钠凝胶电泳分离后根据生物学活性鉴定蛋白质：人外分泌胰腺蛋白质分析
Anal Biochem. 1981 Apr;112(2):304-13. doi: 10.1016/0003-2697(81)90298-0.
6
Gel gradient electrophoresis, isoelectric focusing and two-dimensional techniques in horizontal, ultrathin polyacrylamide layers.凝胶梯度电泳、等电聚焦以及水平超薄聚丙烯酰胺层中的二维技术。
J Biochem Biophys Methods. 1980 Nov;3(5):273-84. doi: 10.1016/0165-022x(80)90008-1.
7
Genetic analysis of human deoxyribonuclease I by immunoblotting and the zymogram method following isoelectric focusing.采用免疫印迹法和等电聚焦后的酶谱法对人脱氧核糖核酸酶I进行遗传分析。
Anal Biochem. 1989 Nov 15;183(1):84-8. doi: 10.1016/0003-2697(89)90175-9.
8
Two-dimensional electrophoresis of the alpha-chains of collagens and its application to the determination of their isoelectric points.胶原蛋白α链的二维电泳及其在测定其等电点中的应用。
Coll Relat Res. 1981 Apr;1(3):237-46. doi: 10.1016/s0174-173x(81)80001-5.
9
Microdisc-electrophoretic study of deoxyribonucleases in cow snout epidermis.牛鼻表皮中脱氧核糖核酸酶的微量圆盘电泳研究。
Arch Dermatol Res. 1983;275(4):213-7. doi: 10.1007/BF00416662.
10
The prevention of distortion in ultrathin-layer polyacrylamide gel isoelectric focusing.超薄层聚丙烯酰胺凝胶等电聚焦中畸变的预防
Anal Biochem. 1984 Apr;138(1):52-6. doi: 10.1016/0003-2697(84)90767-x.

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Fish Physiol Biochem. 1990 Nov;8(6):529-39. doi: 10.1007/BF00003411.
2
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Chicken deoxyribonuclease: purification, characterization, gene cloning and gene expression.
鸡脱氧核糖核酸酶：纯化、特性鉴定、基因克隆与基因表达
J Protein Chem. 2003 Jan;22(1):41-9. doi: 10.1023/a:1023015810840.
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Therapeutic effect of Gag-nuclease fusion protein on retrovirus-infected cell cultures.Gag-核酸酶融合蛋白对逆转录病毒感染细胞培养物的治疗效果。
J Virol. 1996 Jul;70(7):4329-37. doi: 10.1128/JVI.70.7.4329-4337.1996.
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Inhibition of glucosyltransferase activities of Streptococcus mutans by a monoclonal antibody to a subsequence peptide.抗亚序列肽单克隆抗体对变形链球菌葡糖基转移酶活性的抑制作用
Infect Immun. 1993 Nov;61(11):4689-95. doi: 10.1128/iai.61.11.4689-4695.1993.
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Human serum deoxyribonuclease I (DNase I) polymorphism: pattern similarities among isozymes from serum, urine, kidney, liver, and pancreas.人血清脱氧核糖核酸酶I（DNase I）多态性：血清、尿液、肾脏、肝脏和胰腺中同工酶的模式相似性。
Am J Hum Genet. 1990 Jul;47(1):121-6.