Ameliushkina V A, Bochkova N A, Cherniad'eva I F, Titov V N
Lab Delo. 1990(3):46-50.
A specific and sensitive rapid method for simultaneous electrophoretic separation of the blood serum creatine kinase (CK) and lactate dehydrogenase (LDH) isoenzymes is described, fit for rapid diagnosis. A common electrophoresis scheme was used for the separation of both enzymes on the same cellulose acetate plate. Then the isoenzymes were separately detected by their enzymic activities. To detect the enzymic activities at the sites of isoenzymes' localization, the samples were incubated with substrate gel. Optimal conditions for the detection of CK and LDH activities were defined. To illustrate the diagnostic value of the method, the blood serum CK and LDH isoenzymic activities were measured in the patients with acute myocardial infarction and in those operated on for aortocoronary shunting.
本文描述了一种用于同时电泳分离血清肌酸激酶（CK）和乳酸脱氢酶（LDH）同工酶的特异、灵敏的快速方法，适用于快速诊断。采用一种常见的电泳方案，在同一块醋酸纤维素板上分离这两种酶。然后通过酶活性分别检测同工酶。为了在同工酶定位位点检测酶活性，将样品与底物凝胶孵育。确定了检测CK和LDH活性的最佳条件。为了说明该方法的诊断价值，对急性心肌梗死患者和接受主动脉冠状动脉分流手术的患者测定了血清CK和LDH同工酶活性。
