Reppin R, Urbahn H
Z Med Lab Diagn. 1989;30(6):304-7.
Simple and sensitive methods for electrophoretic separation of diagnostic useful CK- and LDH-isoenzymes on cellulose acetate strips are described. The CK-bands were visualized with a commercial CK reagent using the fluorescence of NADPH as an indicator. The optimized reagents for LDH-isoenzymes detection contain a tetrazolium salt for staining the bands. Quantitative scanning of pherograms is possible.
本文描述了在醋酸纤维素条带上对诊断有用的肌酸激酶(CK)和乳酸脱氢酶(LDH)同工酶进行电泳分离的简单且灵敏的方法。CK条带通过使用市售CK试剂,以还原型辅酶Ⅱ(NADPH)的荧光作为指示剂进行可视化。用于检测LDH同工酶的优化试剂含有一种用于染色条带的四唑盐。对电泳图谱进行定量扫描是可行的。