Jarvius Jonas, Melin Jonas, Göransson Jenny, Stenberg Johan, Fredriksson Simon, Gonzalez-Rey Carlos, Bertilsson Stefan, Nilsson Mats
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, 751 85 Uppsala, Sweden.
Nat Methods. 2006 Sep;3(9):725-7. doi: 10.1038/nmeth916.
We describe a scheme for biomolecule enumeration by converting nanometer-scale specific molecular recognition events mediated by rolling-circle amplification to fluorescent micrometer-sized DNA molecules amenable to discrete optical detection. Our amplified single-molecule detection (SMD) approach preserves the discrete nature of the molecular population, allowing multiplex detection and highly precise quantification of molecules over a dynamic range of seven orders of magnitude. We apply the method for sensitive detection and quantification of the bacterial pathogen Vibrio cholerae.
我们描述了一种生物分子计数方案，该方案通过将滚环扩增介导的纳米级特异性分子识别事件转化为适合离散光学检测的荧光微米级DNA分子来实现。我们的扩增单分子检测（SMD）方法保留了分子群体的离散性质，允许在七个数量级的动态范围内对分子进行多重检测和高精度定量。我们将该方法应用于细菌病原体霍乱弧菌的灵敏检测和定量。
