Leonardi A, Briggs R M, Bloch K J, Allansmith M R
Immunology Unit, Eye Research Institute, Boston, Mass.
Ophthalmic Res. 1990;22(2):95-105. doi: 10.1159/000267007.
A model of topically induced ocular anaphylaxis in guinea pigs was developed. A guinea pig anti-dinitrophenyl (DNP) bovine gamma-globulin antiserum rich in IgG1 antibodies was injected subconjunctivally; various serum dilutions and latent periods were tested. The anaphylactic response was elicited with di-DNP-lysine applied topically. The response was characterized by rating 11 ocular signs. Although the 4-hour latent period is considered optimal for passive cutaneous anaphylaxis, a 14-hour latent period yielded the strongest ocular reaction and appeared to the most appropriate time for clinical assessment. Both an early-phase reaction (peaking in the 21st hour) and a late-phase reaction (peaking at 6-10 h) were observed. The two signs of the early-phase reaction that recurred in the late-phase reaction were periorbital swelling and lid redness. The anaphylactic reaction displayed different patterns in different animals: protracted, biphasic and multiphasic.
建立了豚鼠局部诱导性眼过敏模型。将富含IgG1抗体的豚鼠抗二硝基苯基（DNP）牛γ球蛋白抗血清结膜下注射；测试了不同的血清稀释度和潜伏期。局部应用二-DNP-赖氨酸引发过敏反应。通过对11种眼部体征进行评分来表征该反应。尽管4小时潜伏期被认为是被动皮肤过敏反应的最佳时间，但14小时潜伏期产生了最强的眼部反应，似乎是临床评估的最合适时间。观察到了早期反应（在第21小时达到峰值）和晚期反应（在6-10小时达到峰值）。晚期反应中再次出现的早期反应的两个体征是眶周肿胀和眼睑发红。过敏反应在不同动物中表现出不同的模式：持续性、双相性和多相性。
