Leonardi A, Bloch K J, Briggs R, Allansmith M R
Department of Ophthalmology, Harvard Medical School, Boston, Mass.
Ophthalmic Res. 1990;22(4):209-19. doi: 10.1159/000267025.
The ocular late-phase reaction (LPR) is a mast cell-dependent, delayed inflammatory reaction developing 4-12 h after the early-phase reaction (EPR). We developed a passive IgG1 antibody-dependent guinea pig model that clinically reproduced the biphasic reaction of ocular EPR and LPR. An EPR was observed in all animals; a biphasic, multiphasic or prolonged inflammation was observed in the animals maintained for 9 and 24 h. The substantia propria of eyes undergoing EPR (0.5 h) showed intense edema, mast cell degranulation (88%), and 4-fold increase in eosinophils. At 9 h, the neutrophils and eosinophils had increased 11- and 25-fold, respectively. The number of basophils and lymphocytes was significantly increased compared to the controls (p less than 0.05). Of the mast cells, 45% were degranulated compared with 19% for controls. Cellular reactions had subsided by 24 h. Conjunctival epithelium also accumulated inflammatory cells as did the stroma of the lid skin. These histologic changes in ocular tissues undergoing anaphylaxis demonstrated that mast cell degranulation was most severe in the early phase, whereas neutrophil, eosinophil, and basophil accumulation was most marked in the late phase.
眼部迟发相反应（LPR）是一种依赖肥大细胞的迟发性炎症反应，在早发相反应（EPR）后4 - 12小时出现。我们建立了一种被动IgG1抗体依赖的豚鼠模型，该模型临床上重现了眼部EPR和LPR的双相反应。所有动物均观察到EPR；在维持9小时和24小时的动物中观察到双相、多相或延长的炎症反应。经历EPR（0.5小时）的眼固有层显示出强烈水肿、肥大细胞脱颗粒（88%）以及嗜酸性粒细胞增加4倍。在9小时时，中性粒细胞和嗜酸性粒细胞分别增加了11倍和25倍。与对照组相比，嗜碱性粒细胞和淋巴细胞数量显著增加（p小于0.05）。肥大细胞中，45%发生脱颗粒，而对照组为19%。细胞反应在24小时时消退。结膜上皮以及眼睑皮肤基质也积聚了炎症细胞。这些过敏反应中眼部组织的组织学变化表明，肥大细胞脱颗粒在早期最为严重，而中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞的积聚在晚期最为明显。
