Hasenöhrl R U, Gerhardt P, Huston J P
Institute of Physiological Psychology, University of Düsseldorf, F.R.G.
Peptides. 1990 Jan-Feb;11(1):163-7. doi: 10.1016/0196-9781(90)90125-o.
Experiments were performed to investigate the effects of intraperitoneally administered undecapeptide substance P (SP), its N-terminal fragment SP(1-7) (SPN) and the C-terminal analog [pGlu6]-SP(6-11) (SPC) on inhibitory avoidance learning, using a one-trial up-hill avoidance task. In Experiment 1 rats were injected with either SP (50 micrograms/kg), SPN (3.3, 33, 167, 333 micrograms/kg) or SPC (2.7, 27, 134, 268 micrograms/kg) immediately after the training trial. Controls received the diluent vehicles. When tested 24 hr later, rats injected with 50 micrograms/kg SP (37 nmol/kg) and 167 micrograms/kg SPN (185 nmol/kg) exhibited longer step-up latencies than vehicle-treated controls. None of the other doses of SPN nor of the C-terminal fragment influenced performance. In Experiment 2, 167 micrograms/kg SPN or vehicle was injected posttrial either immediately or 5 hr after the training trial. Retention latencies 24 hr later were longer for rats treated with 167 micrograms/kg SPN immediately after the training trial. Performance of the SPN 5-hr delay group did not differ from that of the vehicle-injected controls, ruling out proactive effects of SPN on recall.
采用单次上坡回避任务进行实验，以研究腹腔注射十一肽P物质（SP）、其N端片段SP(1 - 7)（SPN）和C端类似物[pGlu6]-SP(6 - 11)（SPC）对抑制性回避学习的影响。在实验1中，训练试验后立即给大鼠注射SP（50微克/千克）、SPN（3.3、33、167、333微克/千克）或SPC（2.7、27、134、268微克/千克）。对照组注射稀释剂。24小时后进行测试时，注射50微克/千克SP（37纳摩尔/千克）和167微克/千克SPN（185纳摩尔/千克）的大鼠比注射溶剂的对照组表现出更长的上台潜伏期。其他剂量的SPN和C端片段均未影响实验结果。在实验2中，训练试验后立即或5小时后给大鼠注射167微克/千克SPN或溶剂。训练试验后立即注射167微克/千克SPN的大鼠在24小时后的保持潜伏期更长。延迟5小时注射SPN组的实验结果与注射溶剂的对照组无差异，排除了SPN对记忆的前摄作用。
