Babaeva E E, Vorobyova U A, Zharkova M S, Cheknyov S B
Laboratory of Cell-to-Cell Interactions, N F Gamaleya Institute of Epidemiology and Microbiology.
Bull Exp Biol Med. 2006 Jan;141(1):53-6. doi: 10.1007/s10517-006-0092-5.
Binding of copper cations to human serum gamma-globulin was studied using molecular ultrafiltration. The content of free metal in the filtrate was evaluated by the reaction with sodium diethyldithiocarbamate. Conformation characteristics of the protein were evaluated by UV spectrophotometry. gamma-Globulin molecule has several copper-binding sites differing by binding constants and filled one-by-one as the content of bound metal increased.
采用分子超滤法研究了铜阳离子与人血清γ-球蛋白的结合。通过与二乙基二硫代氨基甲酸钠反应评估滤液中游离金属的含量。用紫外分光光度法评估蛋白质的构象特征。γ-球蛋白分子有几个结合常数不同的铜结合位点，随着结合金属含量的增加，这些位点逐一被占据。
