人血清γ-球蛋白与锌阳离子之间的反应。

Reactions between human serum gamma-globulin and zinc cations.

作者信息

Cheknev S B, Babaeva E E, Vorobeva U A, Denisova E A

机构信息

Laboratory of Cell-Cell Interactions, N. F. Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow.

出版信息

Bull Exp Biol Med. 2005 Aug;140(2):209-12. doi: 10.1007/s10517-005-0447-3.

DOI:10.1007/s10517-005-0447-3

PMID:16283003

Abstract

Interactions of human serum gamma-globulin with zinc cations in solution were studied by differential spectrophotometry in UV light. Supraphysiological concentrations of zinc caused an increment in optical density of protein solution reflecting the effect of gamma-globulin saturation with the metal. Zinc concentrations below physiological led to hypochromism in the protein absorption spectrum. Conformation changes in gamma-globulin during interactions with zinc are analyzed for the surface and intramolecular binding sites and are compared with the effects of copper cations.

摘要

采用紫外光差示分光光度法研究了人血清γ-球蛋白与溶液中锌阳离子的相互作用。超生理浓度的锌导致蛋白质溶液的光密度增加，这反映了γ-球蛋白被金属饱和的效应。低于生理浓度的锌导致蛋白质吸收光谱出现减色现象。分析了γ-球蛋白与锌相互作用过程中表面和分子内结合位点的构象变化，并与铜阳离子的作用效果进行了比较。