Vig P J, Mehrotra B D, Desaiah D
Department of Neurology, University of Mississippi Medical Center, Jackson 39216.
Res Commun Chem Pathol Pharmacol. 1990 Mar;67(3):419-22.
The effect of marine toxin, holothurin A, on calmodulin(CaM)-dependent bovine brain 3'-5' phosphodiesterase (PDE) was studied. Holothurin stimulated CaM-depleted PDE in a concentration dependent manner and this interaction was independent of Ca2+ concentrations. Activation of PDE by holothurin was pH and incubation temperature dependent. Heat treatment of holothurin (at 95 degrees C) at various time intervals (2-8 min) decreased its ability to stimulate PDE. CaM-dependent dependent PDE activity was decreased by holothurin. CaM antagonist W-7 and an organochlorine compound chlordecone significantly inhibited holothurin stimulated PDE. These data indicate that holothurin activates CaM-deficient 3'-5' PDE which is antagonized by classical CaM antagonists.
研究了海洋毒素海参毒素A对钙调蛋白（CaM）依赖性牛脑3'-5'磷酸二酯酶（PDE）的影响。海参毒素以浓度依赖性方式刺激CaM缺失的PDE，且这种相互作用与Ca2+浓度无关。海参毒素对PDE的激活作用依赖于pH值和孵育温度。在不同时间间隔（2 - 8分钟）对海参毒素进行热处理（95摄氏度）会降低其刺激PDE的能力。CaM依赖性PDE活性被海参毒素降低。CaM拮抗剂W - 7和有机氯化合物开蓬显著抑制海参毒素刺激的PDE。这些数据表明，海参毒素激活CaM缺陷的3'-5' PDE，而经典的CaM拮抗剂可拮抗这种激活作用。
