Iwasa Y
Horm Metab Res. 1982 May;14(5):265-7. doi: 10.1055/s-2007-1018988.
An inhibitory factor for Ca2+ and calmodulin-dependent cyclic nucleotide phosphodiesterase of bovine brain was present in the soluble fraction of Escherichia coli. The factor was heat-stable but trypsin sensitive. The activity of brain phosphodiesterase supported by Ca2+ and calmodulin was inhibited by the factor in a dose dependent manner, but the basal activity was not affected. The inhibition of phosphodiesterase induced by the factor could be abolished by adding large amount of calmodulin, but not by increasing concentration of Ca2+. It was suggested that the factor interacted with calmodulin and thereby inhibited the phosphodiesterase. The factor may be a calmodulin-binding protein in E. coli.
牛脑Ca2+和钙调蛋白依赖性环核苷酸磷酸二酯酶的一种抑制因子存在于大肠杆菌的可溶部分。该因子耐热但对胰蛋白酶敏感。该因子以剂量依赖的方式抑制由Ca2+和钙调蛋白支持的脑磷酸二酯酶的活性，但对基础活性没有影响。通过添加大量钙调蛋白可以消除该因子诱导的磷酸二酯酶抑制作用，但增加Ca2+浓度则不能。提示该因子与钙调蛋白相互作用从而抑制磷酸二酯酶。该因子可能是大肠杆菌中的一种钙调蛋白结合蛋白。
