Kozela Ewa, Popik Piotr
Behavioral Neuroscience, Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland.
Drug Alcohol Depend. 2007 Jan 12;86(2-3):290-3. doi: 10.1016/j.drugalcdep.2006.06.018. Epub 2006 Aug 22.
Behavioral studies indicate that the inhibition of glutamatergic system with antagonists of ionotropic glutamate NMDA receptors (NMDA-Rs) during long term morphine administration inhibits the development of morphine tolerance and dependence. In the present study we investigated whether chronic morphine treatment leading to the development of morphine antinociceptive tolerance as observed in tail-flick test in mice, may affect the expression of NMDA-R subunits. The expression of NMDA-R subunits was examined in brain areas mediating nociceptive signaling and responsible for antinociceptive activity of morphine. These included periaqueductal grey matter (PAG) and rostral ventromedial medulla (RVM). The expression of NR1 and all subunits of NR2 family (NR2A, NR2B, NR2C, NR2D) of NMDA receptor complex was examined using immunoblotting method. Although behavioral test indicated that morphine antinociceptive tolerance developed, changes in the expression of NMDA-R subunits were not observed, either in the PAG or in the RVM.
行为学研究表明，在长期给予吗啡期间，使用离子型谷氨酸NMDA受体（NMDA-Rs）拮抗剂抑制谷氨酸能系统，可抑制吗啡耐受性和依赖性的发展。在本研究中，我们调查了导致小鼠甩尾试验中出现吗啡镇痛耐受性的慢性吗啡治疗，是否会影响NMDA-R亚基的表达。在介导伤害性信号传导并负责吗啡镇痛活性的脑区中检测了NMDA-R亚基的表达。这些脑区包括中脑导水管周围灰质（PAG）和延髓头端腹内侧（RVM）。使用免疫印迹法检测了NMDA受体复合物的NR1以及NR2家族所有亚基（NR2A、NR2B、NR2C、NR2D）的表达。尽管行为学试验表明吗啡镇痛耐受性已经形成，但在PAG或RVM中均未观察到NMDA-R亚基表达的变化。
