Ray D, Culine S, Tavitain A, Moreau-Gachelin F
INSERM U.248, Faculté de Médecine Lariboisière-Saint-Louis, Paris, France.
Oncogene. 1990 May;5(5):663-8.
Spi-1 is a putative proto-oncogene involved in murine virus-induced acute erythroleukemias. We report here the identification of the human homologue of Spi-1 and its expression in normal and tumorigenic human tissues. Characterization of cDNA clones revealed that the human Spi-1 gene encodes a 216 amino acids protein showing 85% identity with the murine counterpart. By sequencing genomic clones, five exons were identified. To investigate the possible role of Spi-1 gene in human cancers, we studied its expression in a panel of human tumors by Northern blot analysis. Spi-1 expression was detected in all the tumors examined. There was no noticeable evidence of messenger RNA alteration as compared to normal tissues.
Spi-1是一种推定的原癌基因，参与鼠类病毒诱导的急性红白血病。我们在此报告人类Spi-1同源物的鉴定及其在正常和致瘤性人类组织中的表达。cDNA克隆的特征分析表明，人类Spi-1基因编码一种216个氨基酸的蛋白质，与鼠类对应物具有85%的同一性。通过对基因组克隆进行测序，鉴定出了五个外显子。为了研究Spi-1基因在人类癌症中的可能作用，我们通过Northern印迹分析研究了它在一组人类肿瘤中的表达。在所检测的所有肿瘤中均检测到Spi-1表达。与正常组织相比，没有明显的信使RNA改变的证据。
