Moreau-Gachelin F, Ray D, Mattei M G, Tambourin P, Tavitian A
INSERM U-248, Faculté de Médecine Lariboisière Saint Louis, Paris, France.
Oncogene. 1989 Dec;4(12):1449-56.
We have shown previously that spleen focus forming virus integration near the Spi-1 putative oncogene is observed in 95% of erythroid Friend tumors (Moreau-Gachelin et al., 1988). Here we describe how the proviral insertion in the Spi-1 domain is associated with the enhanced transcription of a 1.4 kb mRNA normally expressed at a low level in normal cells. The gene is localized on murine chromosome 2, band E3. The structure of the Spi-1 gene was determined by sequencing genomic and cDNA clones. The gene has an open reading frame encoding a protein of 218 amino acids, extending over five exons. Proviruses always integrate outside, upstream and in the opposite orientation of the protein-encoding domain. This suggests that SFFV integration activates the expression of Spi-2 gene that may contribute to the erythroleukemic process.
我们之前已经表明，在95%的红系Friend肿瘤中可观察到脾集落形成病毒整合在Spi-1假定癌基因附近（莫罗-加谢兰等人，1988年）。在此我们描述了Spi-1结构域中的前病毒插入如何与一种1.4 kb mRNA的转录增强相关，该mRNA在正常细胞中通常低水平表达。该基因定位于小鼠2号染色体E3带。通过对基因组和cDNA克隆进行测序确定了Spi-1基因的结构。该基因有一个开放阅读框，编码一个218个氨基酸的蛋白质，跨越五个外显子。前病毒总是整合在蛋白质编码结构域之外、上游且方向相反。这表明SFFV整合激活了Spi-2基因的表达，这可能有助于红白血病进程。
