Berenson J R, Lichtenstein A, Hart S, Palomares D, Miller R A
Department of Medicine, Wadsworth Cancer Center, UCLA School of Medicine 90024.
Blood. 1990 Jun 1;75(11):2107-11.
Twenty-nine murine monoclonal antibodies have been produced that react with shared idiotypes expressed by B-cell lymphomas and leukemias. We tested this panel of antibodies for reactivity with the paraproteins from 32 patients with multiple myeloma and 10 patients with monoclonal gammopathy of undetermined significance (MGUS). Thirteen of 42 paraproteins reacted with at least one antibody in this panel of anti-idiotypic antibodies. Six different anti-idiotypes demonstrated reactivity with the paraproteins. A similar frequency of reactivity was found for both myeloma and MGUS proteins. One antibody, S30-47, reacted with 6 of 32 (19%) of the paraproteins from patients with multiple myeloma, whereas this anti-idiotype only bound to 3% of non-Hodgkin's B-cell lymphomas and no cases of chronic lymphocytic leukemia. This anti-idiotype reacted with both components of a biphenotypic paraprotein (IgG kappa and IgG lambda) in one patient. In each of nine patients tested, plasma cells isolated from bone marrow were shown to be reactive with the same anti-idiotype we found to react with the paraprotein. Antishared idiotype antibodies may provide useful reagents for studies of patients with monoclonal gammopathies.
已产生29种鼠源单克隆抗体，它们可与B细胞淋巴瘤和白血病所表达的共同独特型发生反应。我们检测了这组抗体与32例多发性骨髓瘤患者及10例意义未明的单克隆丙种球蛋白病（MGUS）患者的副蛋白的反应性。42种副蛋白中有13种与这组抗独特型抗体中的至少一种抗体发生反应。六种不同的抗独特型抗体显示出与这些副蛋白有反应性。骨髓瘤蛋白和MGUS蛋白的反应频率相似。一种抗体S30 - 47与32例多发性骨髓瘤患者中的6例（19%）的副蛋白发生反应，而这种抗独特型抗体仅与3%的非霍奇金B细胞淋巴瘤结合，且未与慢性淋巴细胞白血病病例结合。在一名患者中，这种抗独特型抗体与双表型副蛋白（IgG κ和IgG λ）的两种成分都发生反应。在检测的9名患者中，从骨髓分离出的浆细胞均显示与我们发现能与副蛋白发生反应的同一种抗独特型抗体有反应。抗共同独特型抗体可能为单克隆丙种球蛋白病患者的研究提供有用的试剂。
