Wittmann-Liebold Brigitte, Graack Hanns-Rüdiger, Pohl Thomas
Wittmann Institute of Technology and Analysis of Biomolecules, WITA, Teltow, Berlin, Germany.
Proteomics. 2006 Sep;6(17):4688-703. doi: 10.1002/pmic.200500874.
The proteome analysis by 2-DE is one of the most potent methods of analyzing the complete proteome of cells, cell lines, organs and tissues in proteomics studies. It allows a fast overview of changes in cell processes by analysis of the entire protein extracts in any biological and medical research projects. New instrumentation and advanced technologies provide proteomics studies in a wide variety of biological and biomedical questions. Proteomics work is being applied to study antibiotics-resistant strains and human tissues of various brain, lung, and heart diseases. It cumulated in the identification of antigens for the design of new vaccines. These advances in proteomics have been possible through the development of advanced high-resolution 2-DE systems allowing resolution of up to 10 000 protein spots of entire cell lysates in combination with protein identification by new highly sensitive mass spectrometric techniques. The present technological achievements are suited for a high throughput screening of different cell situations. Proteomics may be used to investigate the health effects of radiation and electromagnetic field to clarify possible dangerous alterations in human beings.
在蛋白质组学研究中，二维电泳（2-DE）蛋白质组分析是分析细胞、细胞系、器官和组织完整蛋白质组的最有效方法之一。通过分析任何生物和医学研究项目中的全蛋白提取物，它能快速概览细胞过程中的变化。新仪器和先进技术为各种各样的生物学和生物医学问题提供了蛋白质组学研究方法。蛋白质组学工作正被应用于研究抗生素抗性菌株以及患有各种脑部、肺部和心脏疾病的人类组织。这一研究积累了用于设计新疫苗的抗原识别成果。通过开发先进的高分辨率二维电泳系统，结合新型高灵敏度质谱技术进行蛋白质鉴定，使得在全细胞裂解物中能够分辨多达10000个蛋白点，蛋白质组学才取得了这些进展。目前的技术成果适用于对不同细胞情况进行高通量筛选。蛋白质组学可用于研究辐射和电磁场对健康的影响，以阐明人类可能出现的危险变化。
