Kalyan Koti, Basu Debdatta, Soundararaghavan Jayanthi
Department of Pathology, Jawahar Lal Institute of Postgraduate Medical Education and Research, Pondicherry.
Indian J Pathol Microbiol. 2006 Apr;49(2):173-7.
Based on morphological, phenotypic, genotypic and clinical findings, Hodgkin's disease has recently been classified into two subtypes: Nodular lymphocyte predominant and classical Hodgkin's disease. Forty-two cases of Hodgkin's disease were subjected to detailed morphological assessment and immunophenotyping. The commonest subtype was Nodular Sclerosis seen in 27 cases. The panel of antibodies used was CD 15, CD 30, CD 3, CD 20, CD 45 and Epithelial Membrane Antigen. Immunophenotyping was done by Streptavidin Biotin Peroxidase complex technique. CD 30 was expressed in 86% and CD 15 in 76% cases. Immunophenotype helped reclassify two cases, according to the WHO classification scheme. Although morphology remains the gold standard in the diagnosis of Hodgkin's disease, immunophenotype is a useful adjunct in differentiating prognostically distinct subtypes.
基于形态学、表型、基因型和临床发现，霍奇金淋巴瘤最近被分为两个亚型：结节性淋巴细胞为主型和经典型霍奇金淋巴瘤。对42例霍奇金淋巴瘤患者进行了详细的形态学评估和免疫表型分析。最常见的亚型是结节硬化型，共27例。所使用的抗体组合包括CD15、CD30、CD3、CD20、CD45和上皮膜抗原。免疫表型分析采用链霉亲和素生物素过氧化物酶复合物技术。86%的病例表达CD30，76%的病例表达CD15。根据世界卫生组织分类方案，免疫表型有助于对两例病例进行重新分类。尽管形态学仍然是霍奇金淋巴瘤诊断的金标准，但免疫表型在区分预后不同的亚型方面是一种有用的辅助手段。
