将含二酮的氨基酸基因导入蛋白质中。
Genetic introduction of a diketone-containing amino acid into proteins.
作者信息
Zeng Huaqiang, Xie Jianming, Schultz Peter G
机构信息
Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
出版信息
Bioorg Med Chem Lett. 2006 Oct 15;16(20):5356-9. doi: 10.1016/j.bmcl.2006.07.094. Epub 2006 Aug 24.
An orthogonal tRNA/aminoacyl-tRNA synthetase pair was evolved that makes possible the site-specific incorporation of an unnatural amino acid bearing a beta-diketone side chain into proteins in Escherichia coli with high translational efficiency and fidelity. Proteins containing this unnatural amino acid can be efficiently and selectively modified with hydroxylamine derivatives of fluorophores and other biophysical probes.
进化出了一对正交的tRNA/氨酰-tRNA合成酶，使得在大肠杆菌中能够以高翻译效率和保真度将带有β-二酮侧链的非天然氨基酸位点特异性地掺入蛋白质中。含有这种非天然氨基酸的蛋白质可以用荧光团的羟胺衍生物和其他生物物理探针进行高效且选择性的修饰。
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