向遗传密码中添加新的化学物质。
Adding new chemistries to the genetic code.
机构信息
The Scripps Research Institute, La Jolla, California 92037, USA.
出版信息
Annu Rev Biochem. 2010;79:413-44. doi: 10.1146/annurev.biochem.052308.105824.
Abstract
The development of new orthogonal aminoacyl-tRNA synthetase/tRNA pairs has led to the addition of approximately 70 unnatural amino acids (UAAs) to the genetic codes of Escherichia coli, yeast, and mammalian cells. These UAAs represent a wide range of structures and functions not found in the canonical 20 amino acids and thus provide new opportunities to generate proteins with enhanced or novel properties and probes of protein structure and function.
摘要
新型正交氨酰-tRNA 合成酶/tRNA 对的发展使约 70 种非天然氨基酸（UAAs）被添加到大肠杆菌、酵母和哺乳动物细胞的遗传密码中。这些 UAA 代表了广泛的结构和功能，而这些在典型的 20 种氨基酸中是找不到的，因此为生成具有增强或新颖特性的蛋白质以及研究蛋白质结构和功能的探针提供了新的机会。
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