Maines Jason, Khurana Neerja R, Roman Karla, Knaup Deborah, Ahmad Mansur
Division of Endodontics, University of Minnesota School of Dentistry, Minneapolis, MN 55455, USA.
J Endod. 2006 Sep;32(9):886-9. doi: 10.1016/j.joen.2006.03.006. Epub 2006 Jun 23.
Although sodium hypochlorite is routinely used as an irrigant, the scope and limitation of another hypohalide sodium hypobromite has not been adequately studied. The objective of this study was to compare the cytotoxic effects of sodium hypobromite and sodium hypochlorite on human osteoblasts by evaluating cell proliferation. One day after plating and every other day after that, cells were treated with a combination of sodium hypochlorite and sodium bromide, resulting into three molar ratios of activated sodium hypobromite. Positive control cells were not treated with hypohalides, while negative controls were separately treated with hypochlorite or bromide alone. Photomicrographic analysis of the cultures was done on days 1 and 6 of treatment. Trypsinized cells were counted to determine cell proliferation. Our results show that sodium bromide is more cytotoxic compared to sodium hypochlorite or activated sodium hypobromite. In activated sodium hypobromite, 4:1 molar ratio was less cytotoxic compared to other molar ratios.
尽管次氯酸钠常被用作冲洗剂，但另一种次卤酸盐——次溴酸钠的作用范围和局限性尚未得到充分研究。本研究的目的是通过评估细胞增殖来比较次溴酸钠和次氯酸钠对人成骨细胞的细胞毒性作用。接种一天后及之后每隔一天，用次氯酸钠和溴化钠的组合处理细胞，从而得到三种摩尔比的活性次溴酸钠。阳性对照细胞不接受次卤酸盐处理，而阴性对照分别单独用次氯酸盐或溴化物处理。在处理的第1天和第6天对培养物进行显微照相分析。对胰蛋白酶消化后的细胞进行计数以确定细胞增殖情况。我们的结果表明，与次氯酸钠或活性次溴酸钠相比，溴化钠的细胞毒性更强。在活性次溴酸钠中，4:1摩尔比的细胞毒性低于其他摩尔比。
