气单胞菌属对四种不同细胞系细胞毒性的比较研究。

Comparative study of cytotoxicity of Aeromonas spp. on four different cell lines.

作者信息

Ghatak S, Agarwal R K, Bhilegaonkar K N

机构信息

Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, India.

出版信息

Comp Immunol Microbiol Infect Dis. 2006 Jul;29(4):233-41. doi: 10.1016/j.cimid.2006.06.005. Epub 2006 Aug 28.

DOI:10.1016/j.cimid.2006.06.005

PMID:16935331

Abstract

In vitro cytotoxicity is an important virulence property of motile mesophilic Aeromonas species. Cell-free supernatant prepared from 55 Aeromonas isolates including one A. hydrophila type strain (MTCC 646) were examined for their cytotoxic potential on four different cell lines (Vero, BHK-21, MDBK, B 95a). Results of the study revealed cytotoxic potential in 92.72% of the isolates. Analysis of data exposed significant variation among isolates in respect of their cytotoxicity. Vero cells proved to be most sensitive to aeromonal toxins and B 95a cells showed significantly (P<0.01) lower response compared to other cell lines. Sensitivities of BHK-21 and MDBK cell lines were in between Vero and B 95a.

摘要

体外细胞毒性是运动性嗜温气单胞菌属的一种重要毒力特性。对从55株气单胞菌分离株（包括一株嗜水气单胞菌标准菌株（MTCC 646））制备的无细胞上清液进行检测，以考察其对四种不同细胞系（Vero、BHK-21、MDBK、B 95a）的细胞毒性潜力。研究结果显示，92.72%的分离株具有细胞毒性潜力。数据分析表明，分离株之间在细胞毒性方面存在显著差异。Vero细胞对气单胞菌毒素最为敏感，与其他细胞系相比，B 95a细胞的反应显著较低（P<0.01）。BHK-21和MDBK细胞系的敏感性介于Vero细胞和B 95a细胞之间。