Molecular Biology Unit, National Centre for Cell Science, Ganeshkhind, Pune, Maharashtra, India.
BMC Microbiol. 2011 Jun 27;11:152. doi: 10.1186/1471-2180-11-152.
Lactobacillus plantarum is considered as a safe and effective probiotic microorganism. Among various sources of isolation, traditionally fermented foods are considered to be rich in Lactobacillus spp., which can be exploited for their probiotic attribute. Antibacterial property of L. plantarum has been demonstrated against various enteric pathogens in both in vitro and in vivo systems. This study was aimed at characterizing L. plantarum isolated from Kutajarista, an ayurvedic fermented biomedicine, and assessing its antagonistic property against a common enteropathogen Aeromonas veronii.
We report the isolation of L. plantarum (VR1) from Kutajarista, and efficacy of its cell free supernatant (CFS) in amelioration of cytotoxicity caused by Aeromonas veronii. On the part of probiotic attributes, VR1 was tolerant to pH 2, 0.3% bile salts and simulated gastric juice. Additionally, VR1 also exhibited adhesive property to human intestinal HT-29 cell line. Furthermore, CFS of VR1 was antibacterial to enteric pathogens like Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Aeromonas veronii and clinical isolates of P. aeruginosa and E. coli. Detailed study regarding the effect of VR1 CFS on A. veronii cytotoxicity showed a significant decrease in vacuole formation and detrimental cellular changes in Vero cells. On the other hand, A. veronii CFS caused disruption of tight junction proteins ZO-1 and actin in MDCK cell line, which was prevented by pre-incubation with CFS of VR1.
This is the first study to report isolation of L. plantarum (VR1) from Kutajarista and characterisation for its probiotic attributes. Our study demonstrates the antagonistic property of VR1 to A. veronii and effect of VR1 CFS in reduction of cellular damage caused by A. veronii in both Vero and MDCK cell lines.
植物乳杆菌被认为是一种安全有效的益生菌微生物。在各种分离源中，传统发酵食品被认为富含乳酸菌属，可以利用其益生菌特性。植物乳杆菌对各种肠道病原体的体外和体内系统均具有抗菌作用。本研究旨在对从 Kutajarista 中分离出的植物乳杆菌进行特征描述，Kutajarista 是一种阿育吠陀发酵生物医学，评估其对常见肠道病原体维罗纳气单胞菌的拮抗特性。
我们从 Kutajarista 中分离出植物乳杆菌（VR1），并评估其无细胞上清液（CFS）对维罗纳气单胞菌引起的细胞毒性的改善作用。就益生菌特性而言，VR1 能耐受 pH2、0.3%胆盐和模拟胃液。此外，VR1 还表现出对人肠 HT-29 细胞系的黏附特性。此外，VR1 的 CFS 对铜绿假单胞菌、金黄色葡萄球菌、大肠杆菌、维罗纳气单胞菌和铜绿假单胞菌和大肠杆菌的临床分离株等肠道病原体具有抗菌作用。关于 VR1 CFS 对维罗纳气单胞菌细胞毒性影响的详细研究表明，空泡形成和 Vero 细胞的有害细胞变化显著减少。另一方面，维罗纳气单胞菌 CFS 破坏了 MDCK 细胞系中的紧密连接蛋白 ZO-1 和肌动蛋白，而 VR1 的 CFS 可通过预孵育来防止这种破坏。
这是首次从 Kutajarista 中分离出植物乳杆菌（VR1）并对其益生菌特性进行特征描述的研究。我们的研究表明，VR1 对维罗纳气单胞菌具有拮抗作用，VR1 的 CFS 可减少维罗纳气单胞菌对 Vero 和 MDCK 细胞系造成的细胞损伤。
