Moon Jun-Woong, Song Yun-Kyoung, Jee Jun-Pil, Kim Chong-Kook, Choung Ho-Kyung, Hwang Jeong-Min
Department of Ophthalmology, Seoul National University College of Medicine, Artificial Eye Center of Clinical Research Institute, Seoul National University Hospital, Seoul, Korea.
Invest Ophthalmol Vis Sci. 2006 Sep;47(9):3968-74. doi: 10.1167/iovs.05-1345.
To investigate the effect of subconjunctival injection of liposome-bound, low-molecular-weight heparin (LMWH) on the absorption rate of subconjunctival hemorrhages.
Subconjunctival hemorrhages were induced in both eyes of 30 rabbits by the subconjunctival injection of 0.1 mL of autologous blood from auricular marginal veins. After 8 hours, randomized subconjunctival injections of one of three materials were made: 5 IU/mL liposome-bound LMWH (0.1 mL) in 18 eyes (group A), only liposomes (0.1 mL) in 14 eyes (group B), the free form of LMWH (5 IU/mL, 0.1 mL) in 14 eyes (group C), or no injection in 14 eyes (group D). Subconjunctival hemorrhages were photographed with a digital camera at 8, 24, 48, 72, 96, and 120 hours after induction of subconjunctival hemorrhages, sized with an image analyzer, and compared between groups.
Subconjunctival hemorrhages were absorbed faster in group A (liposome-bound LMWH injected) than in with group B (liposome injected). Comparison of groups A and C (free LMWH injected) showed statistical differences in the absorption rates at 96 and 120 hours except at 24, 48, and 72 hours. The mean elapsed time for the complete resorption of subconjunctival hemorrhages was shortest in group A among four groups, whereas group B and the control showed no significant differences. The ocular and systemic absorption of LMWH were significantly lower after injection of the liposome-bound than the free form.
The subconjunctival injection of liposome-bound LMWH appears to enhance subconjunctival hemorrhage absorption in rabbits.
研究结膜下注射脂质体结合的低分子量肝素（LMWH）对结膜下出血吸收速率的影响。
通过结膜下注射0.1 mL来自耳廓边缘静脉的自体血，在30只兔子的双眼诱导结膜下出血。8小时后，随机进行三种材料之一的结膜下注射：18只眼注射5 IU/mL脂质体结合的LMWH（0.1 mL）（A组），14只眼注射仅脂质体（0.1 mL）（B组），14只眼注射游离形式的LMWH（5 IU/mL，0.1 mL）（C组），14只眼不注射（D组）。在结膜下出血诱导后的8、24、48、72、96和120小时，用数码相机拍摄结膜下出血情况，用图像分析仪测量大小，并在组间进行比较。
A组（注射脂质体结合的LMWH）结膜下出血的吸收比B组（注射脂质体）更快。A组和C组（注射游离LMWH）比较显示，除24、48和72小时外，在96和120小时吸收速率有统计学差异。四组中，A组结膜下出血完全吸收的平均时间最短，而B组和对照组无显著差异。注射脂质体结合的LMWH后，其眼部和全身吸收明显低于游离形式。
结膜下注射脂质体结合的LMWH似乎可促进兔结膜下出血的吸收。
