小鼠BCM1（OX45或Blast-1）抗原的结构、表达及遗传连锁。关于基因重复导致小鼠1号染色体上的BCM1区域和小鼠3号染色体上的CD2/LFA3区域产生的证据。
Structure, expression, and genetic linkage of the mouse BCM1 (OX45 or Blast-1) antigen. Evidence for genetic duplication giving rise to the BCM1 region on mouse chromosome 1 and the CD2/LFA3 region on mouse chromosome 3.
作者信息
Wong Y W, Williams A F, Kingsmore S F, Seldin M F
机构信息
Medical Research Council Cellular Immunology Research Unit, Sir William Dunn School of Pathology, University of Oxford, England.
出版信息
J Exp Med. 1990 Jun 1;171(6):2115-30. doi: 10.1084/jem.171.6.2115.
The mouse BCM1 (OX45, Blast-1) antigen has been cDNA cloned and sequenced to provide data supporting the view that BCM1, LFA3, and CD2 constitute a subgroup within the Ig superfamily. Mouse BCM1 is widely expressed on leukocytes and is likely to be anchored to the cell surface by a glycosyl-phosphatidylinositol anchor, as is the case for rat and human BCM1 antigen. Genetic linkage studies by recombination and pulse field analysis showed the BCM1 locus (Bcm-1) to be on distal mouse chromosome 1 and to be linked within 1,600 kb to the locus for an ATPase alpha chain gene (Atpa-3). A similar relationship was established between the human BCM1 locus (BCM1) and ATP1A2, and other markers on chromosome 1q. Conservation of genomic organization within a segment of human chromosome 1q and mouse chromosome 1 was demonstrated. A similar situation is seen in the region of the CD2 and LFA3 genes between mouse chromosome 3 and human chromosome 1p. Furthermore, the CD2/LFA3 genes are linked within 580 kb to Atpa-1/ATP1A1 genes to provide a parallel situation to the linkage between Bcm-1/BCM1 and Atpa-3/ATP1A2 on chromosomes 1 (mouse) and 1q (human). Taken together, the data suggest duplication of a chromosome region including the precursors of the genes for BCM1, CD2, and LFA3, and the ATPase genes to give rise to the linkage groups now observed. The duplicated regions may have stayed together on chromosome 1 in the human (with the insertion of a centromere), while in the mouse, the genetic regions are proposed to have become dispersed in the formation of chromosomes 1 and 3. CD2 and LFA3 are more dissimilar in sequence than BCM1 and LFA3, and if the precursors of the CD2 and LFA3 loci formed before the proposed chromosome segment duplication, then a gene encoding a recognizer molecule for BCM1 may exist in linkage with Bcm-1/BCM1 on chromosome 1 (mouse) and 1q (human).
小鼠BCM1（OX45，Blast-1）抗原已被进行cDNA克隆和测序，以提供数据支持以下观点：BCM1、LFA3和CD2构成免疫球蛋白超家族中的一个亚组。小鼠BCM1在白细胞上广泛表达，并且很可能像大鼠和人类BCM1抗原一样，通过糖基磷脂酰肌醇锚定在细胞表面。通过重组和脉冲场分析进行的遗传连锁研究表明，BCM1基因座（Bcm-1）位于小鼠1号染色体远端，并且在1600 kb范围内与一个ATP酶α链基因（Atpa-3）的基因座连锁。在人类BCM1基因座（BCM1）与ATP1A2以及1号染色体长臂上的其他标记之间也建立了类似的关系。已证明人类1号染色体长臂和小鼠1号染色体的一个片段内基因组组织的保守性。在小鼠3号染色体和人类1号染色体短臂之间的CD2和LFA3基因区域也有类似情况。此外，CD2/LFA3基因在580 kb范围内与Atpa-1/ATP1A1基因连锁，这与1号染色体（小鼠）和1号染色体长臂（人类）上Bcm-1/BCM1与Atpa-3/ATP1A2之间的连锁情况相似。综合来看，这些数据表明包括BCM1、CD2和LFA3基因以及ATP酶基因前体的一个染色体区域发生了重复，从而形成了现在观察到的连锁群。在人类中，重复区域可能在1号染色体上聚集在一起（伴随着一个着丝粒的插入），而在小鼠中，这些遗传区域被认为在染色体1和3的形成过程中分散开来。CD2和LFA3在序列上比BCM1和LFA3更不相似，如果CD2和LFA3基因座的前体在假定的染色体片段重复之前就已形成，那么一个编码BCM1识别分子的基因可能与小鼠1号染色体和人类1号染色体长臂上的Bcm-1/BCM1连锁。
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