Yuan Jie, Fowler William U, Kimball Elizabeth, Lu Wenyun, Rabinowitz Joshua D
Department of Chemistry and Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA.
Nat Chem Biol. 2006 Oct;2(10):529-30. doi: 10.1038/nchembio816. Epub 2006 Aug 27.
We present a new method for probing cellular metabolic fluxes that is based on the kinetics of assimilation of isotope-labeled nutrient into a diversity of downstream metabolites. In the case of nitrogen assimilation, half-maximal labeling of most metabolites occurs in 10-300 s. Fluxes measured on the basis of the kinetics of nitrogen assimilation in exponentially growing E. coli agree well with those fluxes predicted to allow optimal biomass production.
我们提出了一种探测细胞代谢通量的新方法，该方法基于将同位素标记的营养物质同化为多种下游代谢物的动力学。在氮同化的情况下，大多数代谢物的半最大标记在10 - 300秒内发生。基于指数生长的大肠杆菌中氮同化动力学测量的通量与预测的允许最佳生物量产生的通量非常吻合。
