Lima D, Coimbra A
Institute of Histology and Embryology, Faculty of Medicine, University of Oporto, Porto, Portugal.
Neuroscience. 1990;34(3):591-606. doi: 10.1016/0306-4522(90)90167-3.
The morphological features of lamina I neurons labelled from the medullary dorsal reticular nucleus with free or wheat germ agglutinin conjugated horseradish peroxidase and cholera toxin subunit B, were studied in the three standard anatomical planes in the rat. Orientation and way of branching of the dendritic arbors were further analysed by the method of Sholl in cells labelled with cholera toxin subunit B. Most marginal cells belong to the multipolar type (70%) of our Golgi-based classification, and a minority to the pyramidal (15%) and flattened (15%) types. Following unilateral lesions severing the greatest part of the cuneate fasciculus, a considerable decrease of the numbers of labeled cells of the three types was observed caudal and ipsilaterally to the lesion. Contralateral labelling of multipolar and pyramidal cells was less decreased, and that of flattened cells was apparently unchanged. While multipolar cells, which make up the bulk of marginal spinobulbar neurons, appear to have no other supraspinal target, pyramidal and flattened cells have been labelled from the mesencephalon and the thalamus, respectively. It is suggested that the three structural cell types subserve different aspects of the spinofugal nociceptive output.
运用游离或与麦胚凝集素结合的辣根过氧化物酶以及霍乱毒素B亚基，从延髓背侧网状核标记I层神经元，并在大鼠的三个标准解剖平面上研究其形态特征。通过Sholl方法，对用霍乱毒素B亚基标记的细胞的树突分支方向和方式进行了进一步分析。在基于高尔基染色的分类中，大多数边缘细胞属于多极型（70%），少数属于锥体型（15%）和平扁型（15%）。在单侧损伤切断大部分楔束后，在损伤尾侧和同侧观察到这三种类型标记细胞的数量显著减少。多极细胞和锥体细胞的对侧标记减少较少，平扁细胞的对侧标记明显未变。构成边缘脊髓延髓神经元主体的多极细胞似乎没有其他脊髓上靶点，而锥体细胞和平扁细胞分别从中脑和丘脑被标记。提示这三种结构细胞类型在脊髓传出痛觉输出的不同方面发挥作用。
