Panneton W M, Johnson S N, Christensen N D
Department of Anatomy and Neurobiology, St Louis University School of Medicine, MO 63104.
Neuroscience. 1994 Feb;58(3):605-25. doi: 10.1016/0306-4522(94)90085-x.
The anterograde and retrograde transport of wheat germ agglutinin-horseradish peroxidase was used to study the trigeminoperibrachial pathway in the muskrat after injections of tracer into either the medullary dorsal horn or the dorsolateral pons. After injections into the medullary dorsal horn, labeled fibers ascended into the ipsilateral dorsolateral pons via the spinal trigeminal tract, within the neuropil of the trigeminal sensory complex and within the reticular formation adjacent to the spinal trigeminal nucleus. At caudal levels of the ipsilateral peribrachial area, dense terminal-like label distributed in the Kölliker-Fuse nucleus continued into the lateral parabrachial nucleus. At intermediate levels ipsilaterally, the Kölliker-Fuse nucleus again was labeled densely, as were areas analogous to the external lateral and external medial subnuclei of the parabrachial nucleus in the rat. A thin band of label along the ventral spinocerebellar tract outlined an unlabeled area in the central portion of the lateral parabrachial nucleus. Rostrally near the pontomesencephalic junction, the area designated the superior lateral subnucleus in the hamster was labeled, while sparser label was present more dorsally. Contralateral to the injections, caudal and intermediate levels of the peribrachial area contained only scant reaction product. However, the rostral area of the superior lateral subnucleus was labeled densely via fibers ascending in the trigeminothalamic tract. Injections made just rostral to the obex and either centered in or including the dorsal or ventral paratrigeminal nuclei produced similar labeling at caudal and intermediate levels of the peribrachial area. An exception, however, was that the caudal medial parabrachial nucleus was also labeled after the dorsal paratrigeminal injection. Also, only scant label was found in the rostral third of the dorsolateral pons on either side after these injections. Both trigeminothalamic and trigeminolemniscal pathways were labeled contralaterally after these injections. These trigeminal projections to the dorsolateral pons were compared to the projections from the nucleus tractus solitarii and the ventrolateral medulla. Numerous trigeminal neurons were labeled retrogradely after injections of wheat germ agglutinin-horseradish peroxidase into the dorsolateral pons. In the medullary dorsal horn, they were found almost exclusively in laminae I and V. Labeled neurons in lamina I were especially prominent in rostral ventral levels of the medullary dorsal horn. Labeled cells in lamina I were continuous with others found in the displaced band of substantia gelatinosa at the interface of the subnucleus caudalis and subnucleus interpolaris, as well as with those found in the ventral and dorsal paratrigeminal nuclei.(ABSTRACT TRUNCATED AT 400 WORDS)
将小麦胚凝集素 - 辣根过氧化物酶的顺行和逆行运输用于研究麝鼠的三叉臂周通路，方法是将示踪剂注射到延髓背角或脑桥背外侧。将示踪剂注射到延髓背角后，标记纤维通过三叉神经脊髓束、三叉神经感觉复合体的神经毡以及与三叉神经脊髓核相邻的网状结构上升到同侧脑桥背外侧。在同侧臂周区的尾侧水平，分布在 Kölliker - Fuse 核中的密集终末样标记延续到外侧臂旁核。在同侧中间水平，Kölliker - Fuse 核再次被密集标记，大鼠臂旁核的外侧和内侧外侧亚核类似区域也被标记。沿着腹侧脊髓小脑束的一条细标记带勾勒出外侧臂旁核中央部分的一个未标记区域。在脑桥中脑交界处附近的头端，仓鼠中称为上外侧亚核的区域被标记，而在更靠背侧的区域标记较稀疏。与注射部位对侧相比，臂周区的尾侧和中间水平仅含有少量反应产物。然而，上外侧亚核的头端区域通过三叉丘脑束上升的纤维被密集标记。在闩前方且以背侧或腹侧三叉旁核为中心或包含这些核的注射，在臂周区的尾侧和中间水平产生类似的标记。然而，一个例外是，背侧三叉旁核注射后，尾侧内侧臂旁核也被标记。此外，这些注射后，两侧脑桥背外侧头端三分之一处仅发现少量标记。这些三叉神经向脑桥背外侧的投射与孤束核和延髓腹外侧的投射进行了比较。将小麦胚凝集素 - 辣根过氧化物酶注射到脑桥背外侧后，许多三叉神经神经元被逆行标记。在延髓背角，它们几乎只在 I 层和 V 层中被发现。I 层中的标记神经元在延髓背角头端腹侧水平尤为突出。I 层中的标记细胞与在尾侧亚核和极间亚核交界处的胶状质移位带中发现的其他细胞以及在腹侧和背侧三叉旁核中发现的细胞相连。（摘要截短至400字）
