Purcell Scott H, Seidel George E, McCue Patrick M, Squires Edward L
Animal Reproduction and Biotechnology Lab, Colorado State University, Fort Collins, CO 80523-1683, USA.
Anim Reprod Sci. 2007 Aug;100(3-4):291-300. doi: 10.1016/j.anireprosci.2006.07.009. Epub 2006 Aug 30.
The aim of this study was to compare the efficacy of three approaches for recovering equine oocytes via transvaginal ultrasound-guided follicular aspiration. Fourteen mares were used as oocyte donors during the spring transition period and physiologic breeding season, and 11 mares were bred for use as oocyte donors during early gestation. In all mares, large (>20 mm) and small (10-20 mm) follicles were aspirated in eight rounds every 10-11 days. In each of the four rounds during the transition period, half the mares received 12.5 mg eFSH once daily for 4 days prior to aspiration. For each of the four rounds during the cycling season, half the mares received 12.5 mg eFSH twice daily for 3 days prior to aspiration. Pregnant mares were aspirated on days 25, 40 and 55 of gestation and received no eFSH. There were more large (>20 mm) follicles in cycling controls (2.25+/-0.27) and cycling FSH-treated (2.64+/-0.27) mares than in transitional FSH-treated mares (1.18+/-0.27). The number of oocytes recovered from small (10-20 mm) follicles varied by mare (P<0.05) and averaged 1.08+/-0.22 per aspiration for transitional mares and 1.23+/-0.22 per aspiration for cycling mares (P>0.1). The number of oocytes per aspiration from large follicles was greater in cycling FSH-treated mares (0.46+/-0.09) than in transitional control mares (0.11+/-0.09). In pregnant mares, more large follicles were present at day 25 than at any other time, and the number of oocytes per aspiration from large follicles was greater at day 25 (0.73+/-0.16) than at day 55 (0.04+/-0.18). When compared across all seasons and treatments, the day 25 pregnant mares yielded the greatest number of oocytes per aspiration (2.91+/-0.66 per mare).
本研究的目的是比较经阴道超声引导卵泡抽吸术回收马属动物卵母细胞的三种方法的疗效。在春季过渡期和生理繁殖季节，14匹母马被用作卵母细胞供体，在妊娠早期，11匹母马被用于繁殖作为卵母细胞供体。在所有母马中，每10 - 11天进行8轮抽吸，抽吸大（>20 mm）卵泡和小（10 - 20 mm）卵泡。在过渡期的四轮抽吸中，每轮有一半的母马在抽吸前4天每天接受12.5 mg eFSH。在发情周期季节的四轮抽吸中，每轮有一半的母马在抽吸前3天每天接受12.5 mg eFSH两次。妊娠母马在妊娠第25、40和55天进行抽吸，且不接受eFSH。发情周期对照组（2.25±0.27）和发情周期FSH处理组（2.64±0.27）母马中的大（>20 mm）卵泡数量多于过渡期FSH处理组母马（1.18±0.27）。从小（10 - 20 mm）卵泡中回收的卵母细胞数量因母马而异（P<0.05），过渡期母马每次抽吸平均为1.08±0.22个，发情周期母马每次抽吸平均为1.23±0.22个（P>0.1）。发情周期FSH处理组母马从大卵泡中每次抽吸获得的卵母细胞数量（0.46±0.09）多于过渡期对照组母马（0.11±0.09）。在妊娠母马中，第25天的大卵泡数量比其他任何时候都多，且第25天从大卵泡中每次抽吸获得的卵母细胞数量（0.73±0.16）多于第55天（0.04±0.18）。在所有季节和处理方式之间进行比较时，妊娠第25天的母马每次抽吸获得的卵母细胞数量最多（每匹母马2.91±0.66个）。
