Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843-4466, USA.
Theriogenology. 2010 May;73(8):1116-26. doi: 10.1016/j.theriogenology.2010.01.013. Epub 2010 Mar 4.
Oocytes may be collected from live mares from either the stimulated preovulatory follicle or from all visible immature follicles. We evaluated the yield of mature oocytes, and of blastocysts after intracytoplasmic sperm injection (ICSI), for both follicle types. In Experiment 1, mares were assigned to Progesterone (1.2g biorelease progesterone weekly) or Control treatments. Transvaginal aspiration of all follicles was performed every 14 d. Overall, 596 follicles were aspirated, with a 54% oocyte recovery rate. There was no difference between treatments in number of follicles punctured (9.0 to 9.1) or oocytes recovered (4.8 to 5.0) per mare per aspiration session. Of 314 oocytes recovered, 180 (57%) matured in culture. Thirty-six mature oocytes were subjected to ICSI; 33% formed blastocysts (63% per mare per aspiration session). In Experiment 2, the preovulatory follicle was aspirated every 14 d for three to four cycles. Prostaglandin F(2 alpha) was given on Days 6 and 7 after aspiration. A follicle >or=25 mm in diameter was present on Day 13, the day of deslorelin administration, in 23 of 24 cycles, and ovulatory response (granulosa expansion) was seen in 24 of 25 follicles aspirated. Blastocyst development after ICSI was 41% per injected oocyte, or an estimated 33% per mare per aspiration session. We concluded that both aspiration of immature follicles and aspiration of the preovulatory follicle can be performed effectively every 14 d without monitoring ovarian follicular growth. As performed in these separate experiments, aspiration of immature follicles provided more blastocysts per aspiration session.
卵母细胞可以从活马的促排卵前卵泡或所有可见的未成熟卵泡中采集。我们评估了这两种卵泡类型的成熟卵母细胞和胞浆内精子注射（ICSI）后囊胚的产量。在实验 1 中，马被分配到孕激素（每周 1.2g 生物释放孕激素）或对照处理。每隔 14 天进行阴道超声引导下所有卵泡抽吸。总共抽吸了 596 个卵泡，卵母细胞回收率为 54%。两种处理方式之间，每匹马每次抽吸穿刺的卵泡数（9.0 到 9.1）或回收的卵母细胞数（4.8 到 5.0）没有差异。在 314 个回收的卵母细胞中，180 个（57%）在培养中成熟。36 个成熟卵母细胞进行 ICSI；33%形成囊胚（每匹马每次抽吸 63%）。在实验 2 中，每隔 14 天抽吸促排卵前卵泡，共抽吸三个到四个周期。在抽吸后第 6 和第 7 天给予前列腺素 F2α。在 24 个周期中，有 23 个周期在第 13 天（即去势诱导素给药日）出现了直径≥25mm 的卵泡，并且在 25 个抽吸的卵泡中，有 24 个出现了排卵反应（颗粒细胞扩张）。ICSI 后囊胚发育率为每注射卵母细胞 41%，或估计每匹马每次抽吸 33%。我们得出结论，每隔 14 天不监测卵巢卵泡生长，就可以有效地进行未成熟卵泡和促排卵前卵泡的抽吸。在这两个单独的实验中，未成熟卵泡的抽吸每抽吸次数提供了更多的囊胚。
