Fan Jian-Bing, Gunderson Kevin L, Bibikova Marina, Yeakley Joanne M, Chen Jing, Wickham Garcia Eliza, Lebruska Lori L, Laurent Marc, Shen Richard, Barker David
Illumina, Inc., San Diego, California, USA.
Methods Enzymol. 2006;410:57-73. doi: 10.1016/S0076-6879(06)10003-8.
This chapter describes an accurate, scalable, and flexible microarray technology. It includes a miniaturized array platform where each individual feature is quality controlled and a versatile assay that can be adapted for various genetic analyses, such as single nucleotide polymorphism genotyping, DNA methylation detection, and gene expression profiling. This chapter describes the concept of the BeadArray technology, two different Array of Arrays formats, the assay scheme and protocol, the performance of the system, and its use in large-scale genetic, epigenetic, and expression studies.
本章介绍了一种准确、可扩展且灵活的微阵列技术。它包括一个小型化阵列平台，其中每个单独的特征都经过质量控制，以及一种通用检测方法，该方法可适用于各种基因分析，如单核苷酸多态性基因分型、DNA甲基化检测和基因表达谱分析。本章描述了珠阵列技术的概念、两种不同的阵列格式、检测方案和协议、系统性能及其在大规模基因、表观遗传和表达研究中的应用。
