Barnett B C, Hartlmayr I, Graham C M, Thomas D B
National Institute for Medical Research, Mill Hill, London.
Immunology. 1990 May;70(1):48-54.
A majority of Iad-restricted, CD4+ T-cell clones, derived from BALB/c mice infected with X31 (H3N2) influenza virus and specific for the HA 1 subunit of the viral haemagglutinin (HA), has previously been shown to recognize the synthetic peptide HA 1 177-199, corresponding to the primary amino acid sequence of a major antibody binding site. Here it is demonstrated that both I-Ad- and I-Ed-restricted T-cell clones recognize HA 1 177-199, and that inter- and intra-allelic differences in Iad-restricted recognition are defined by single amino acid residues. A panel of truncated HA 1 synthetic peptides defined three distinct but overlapping CD4+ epitopes within the common antigenic site (HA 1 177-199): two I-Ad-restricted epitopes mapped within HA 1 186-198 and HA 1 177-199, and peptide HA 1 178-195 identified an I-Ed-restricted epitope. Moreover, fine specificity differences in the recognition of synthetic peptides, truncated at the carboxy terminus of HA 1 177-199, identified residues HA 1 A 198 and HA 1 S 199 as being critical for defining inter- and intra-allelic differences, respectively, in the Iad-restricted T-cell recognition of HA.
先前已表明，从感染X31（H3N2）流感病毒的BALB/c小鼠中获得的、针对病毒血凝素（HA）的HA 1亚基且受Iad限制的大多数CD4+ T细胞克隆，能够识别合成肽HA 1 177 - 199，该合成肽对应于一个主要抗体结合位点的一级氨基酸序列。本文证明，受I-Ad和I-Ed限制的T细胞克隆均能识别HA 1 177 - 199，并且Iad限制识别中的等位基因间和等位基因内差异由单个氨基酸残基决定。一组截短的HA 1合成肽在共同抗原位点（HA 1 177 - 199）内确定了三个不同但重叠的CD4+表位：两个受I-Ad限制的表位定位于HA 1 186 - 198和HA 1 177 - 199内，肽HA 1 178 - 195确定了一个受I-Ed限制的表位。此外，对在HA 1 177 - 199羧基末端截短的合成肽识别中的精细特异性差异进行分析，确定残基HA 1 A 198和HA 1 S 199分别对于定义Iad限制的T细胞对HA识别中的等位基因间和等位基因内差异至关重要。
