Underwood P A
J Gen Virol. 1982 Sep;62 (Pt 1):153-69. doi: 10.1099/0022-1317-62-1-153.
A panel of 125 monoclonal antibodies (IgG) was raised against the haemagglutinin of an early representative of the Hong Kong (H3N2) subtype of influenza. They were classified into groups based on their cross-reactions with 16 other virus strains from the same subtype. This classification was performed using methods of numerical taxonomy. Statistical tests supported the validity of the grouping. Ten such groups were identified. Nine antibodies remained unclassified. The locations on the haemagglutinin molecule of amino acid residues influencing the binding of each antibody group were estimated. This was achieved by a study of antibody cross-reactive profiles, coupled with previously published locations of amino acid changes in the primary sequence of different haemagglutinins, and their positions in the tertiary structure of the molecule. The locations of the amino acids affecting antibody binding overlapped between the different antibody groups forming a continuous ring surrounding the probable cell-receptor pocket. The amino acids affecting the binding of each antibody group may or may not represent the actual antibody binding sites. The importance of the different sites of amino acid variation in the haemagglutinin during evolution of the virus is discussed.
制备了一组针对甲型流感香港（H3N2）亚型早期代表株血凝素的125种单克隆抗体（IgG）。根据它们与同一亚型的其他16种病毒株的交叉反应进行分组。这种分类采用数值分类学方法进行。统计检验支持分组的有效性。确定了10个这样的组。9种抗体未分类。通过研究抗体交叉反应谱，结合先前发表的不同血凝素一级序列中氨基酸变化的位置及其在分子三级结构中的位置，估计了影响每个抗体组结合的血凝素分子上氨基酸残基的位置。影响抗体结合的氨基酸位置在不同抗体组之间重叠，形成围绕可能的细胞受体口袋的连续环。影响每个抗体组结合的氨基酸可能代表也可能不代表实际的抗体结合位点。讨论了血凝素中氨基酸变异的不同位点在病毒进化过程中的重要性。
