Karagouni E E, Hadjipetrou-Kourounakis L
Department of Genetics, Development, and Molecular Biology, Faculty of Biology, School of Science, Aristotelian University, Thessaloniki, Greece.
Scand J Immunol. 1990 Jun;31(6):745-54. doi: 10.1111/j.1365-3083.1990.tb02826.x.
Mice were immunized against fluorescein isothiocyanate (FITC)-labelled human gamma globulin (HGG) or dextran sulphate (DXS) in the absence or presence of different adjuvants. The immune response was assayed as the total Ig-secreting cells and FITC-specific plaque-forming cells (PFC) found in various lymphoid organs. The adjuvants influenced the isotype of antibodies produced to the same antigenic determinant. The PFC of different IgG subclasses were favoured by different adjuvants. The IgG3 isotype was produced mainly after immunization with either antigen and lipopolysaccharide (LPS) or Li salt as adjuvant; IgG1 was produced with incomplete Freund's adjuvant (IFA), complete Freund's adjuvant (CFA), alum, poly I:C, Quil A, Be salt, and poly A:U. Some of the above adjuvants (Be salt and poly A:U) favoured the production of IgG2b, and others (CFA, alum, Quil A, and poly I:C) favoured the IgG2a isotype besides the main isotype. Attempts were made to correlate the activation by the various adjuvants of certain TH subtypes with the isotypes produced.
在不存在或存在不同佐剂的情况下，用异硫氰酸荧光素（FITC）标记的人γ球蛋白（HGG）或硫酸葡聚糖（DXS）对小鼠进行免疫。以在各种淋巴器官中发现的总Ig分泌细胞和FITC特异性噬斑形成细胞（PFC）来测定免疫反应。佐剂影响针对相同抗原决定簇产生的抗体的同种型。不同佐剂有利于不同IgG亚类的PFC产生。IgG3同种型主要在用抗原和脂多糖（LPS）或锂盐作为佐剂免疫后产生；IgG1在用不完全弗氏佐剂（IFA）、完全弗氏佐剂（CFA）、明矾、聚肌胞苷酸（poly I:C）、商陆丝裂原（Quil A）、铍盐和聚腺苷酸:尿苷酸（poly A:U）免疫后产生。上述一些佐剂（铍盐和poly A:U）有利于IgG2b的产生，而其他一些佐剂（CFA、明矾、Quil A和poly I:C）除了主要同种型外还有利于IgG2a同种型的产生。研究人员试图将某些TH亚型被各种佐剂激活的情况与产生的同种型联系起来。
