Tavangar K, Hoffman A R, Kraemer F B
Division of Endocrinology, Stanford University School of Medicine, California 94305.
Anal Biochem. 1990 Apr;186(1):60-3. doi: 10.1016/0003-2697(90)90572-q.
We have developed a simple and rapid procedure for the isolation of total RNA from small amounts of adipose tissue. Using this method, it is possible to obtain quantitative recovery of RNA from less than 300 mg of adipose tissue, with an average yield of 70 micrograms of RNA per gram of adipose tissue. Northern blot analysis of rat epididymal adipose tissue RNA samples was performed using a beta-actin probe and demonstrated that intact total RNA had been isolated. The procedure has been adapted for use in 1.5-ml microcentrifuge Eppendorf tubes, providing a convenient and inexpensive method for the reproducible recovery of intact RNA from sparse samples of adipose tissue.
我们已经开发出一种从少量脂肪组织中分离总RNA的简单快速方法。使用该方法，能够从少于300毫克的脂肪组织中定量回收RNA，平均每克脂肪组织的RNA产量为70微克。使用β-肌动蛋白探针，对大鼠附睾脂肪组织RNA样本进行了Northern印迹分析，结果表明已分离出完整的总RNA。该方法已适用于1.5毫升微量离心Eppendorf管，为从脂肪组织稀少样本中可重复回收完整RNA提供了一种方便且廉价的方法。
