一种用于RNA凝胶电泳和Northern印迹的简单方法。 - Suppr

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超能文献

A simple procedure for gel electrophoresis and northern blotting of RNA.

作者信息

Goda S K, Minton N P

机构信息

Department of Molecular Microbiology, Centre for Applied Microbiology and Research, Salisbury, Wiltshire, UK.

出版信息

Nucleic Acids Res. 1995 Aug 25;23(16):3357-8. doi: 10.1093/nar/23.16.3357.

DOI:10.1093/nar/23.16.3357

PMID:7545288

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307204/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37c9/307204/54784cb0631c/nar00016-0286-a.jpg

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本文引用的文献

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.变性RNA与转移至硝酸纤维素膜上的小DNA片段的杂交。

Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5. doi: 10.1073/pnas.77.9.5201.

The complete amino acid sequence of the Clostridium botulinum type A neurotoxin, deduced by nucleotide sequence analysis of the encoding gene.通过对编码基因进行核苷酸序列分析推导得出的A型肉毒杆菌神经毒素的完整氨基酸序列。

Eur J Biochem. 1990 Apr 20;189(1):73-81. doi: 10.1111/j.1432-1033.1990.tb15461.x.

Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.甲基汞作为琼脂糖凝胶电泳的可逆变性剂。

Anal Biochem. 1976 Jan;70(1):75-85. doi: 10.1016/s0003-2697(76)80049-8.

RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.变性条件下通过凝胶电泳测定RNA分子量：一项关键的重新审视

Biochemistry. 1977 Oct 18;16(21):4743-51. doi: 10.1021/bi00640a033.

Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA.寡聚（dT）-纤维素色谱法在分离聚腺苷酸化RNA中的特异性

Anal Biochem. 1976 May 7;72:413-27. doi: 10.1016/0003-2697(76)90549-2.

Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels.从甲醛琼脂糖凝胶转移至重氮苄氧基甲基纸上的前胶原信使核糖核酸的鉴定

Nucleic Acids Res. 1979 Aug 10;6(11):3559-67. doi: 10.1093/nar/6.11.3559.

Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.使用乙二醛和吖啶橙对聚丙烯酰胺凝胶和琼脂糖凝胶上的单链和双链核酸进行分析。

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