Iwamura Chiaki, Aihara Michiko, Nakazawa Masatoshi, Takahashi Kazuo, Yoshioka Naoya, Matsukura Setsuko, Hirasawa Tsutomu, Minami Mutsuhiko, Ikezawa Zenro
Department of Environmental Immuno-Dermatology, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
Int Arch Allergy Immunol. 2006;141(4):337-45. doi: 10.1159/000095460. Epub 2006 Aug 29.
In the last few decades, numerous chemical compounds have been produced as a result of industrial development. At the same time, the number of atopic dermatitis (AD) patients has been increasing. It has been reported that tributyltin (TBT) compounds have effects not only on the reproductive system but also on the immune system.
To investigate whether TBT has an effect on AD, we fed a diet containing TBT to DS-Nh mice, which spontaneously developed dermatitis under conventional conditions.
DS-Nh mice fed TBT or a control diet were examined for skin changes, number of Staphylococcus aureus on the skin and serum IgE levels. To determine Th1/Th2 cytokine production by lymphocytes, lymphocytes of DS-Nh mice fed TBT and of controls were cultured with staphylococcal enterotoxin B and cytokine levels in the supernatants were measured by ELISA. We observed not only spontaneous dermatitis but also dermatitis induced by sensitization with 2,4,6-trinitrochlorobenzene (TNCB).
The AD-like lesions induced by TNCB sensitization were more severe in the mice fed TBT than in those fed the control diet. A greater increase in S. aureus on the skin was observed in the mice fed TBT than in the mice fed the control diet. A decrease in IFN-gamma production and an increase in IL-5 and IL-13 production were observed in the mice fed the TBT diet and treated with TNCB. These findings suggest that the increase in S. aureus and the enhancement of Th2 response induced by TBT exacerbate the AD-like lesions in mice treated with TNCB.
在过去几十年中，工业发展产生了大量化合物。与此同时，特应性皮炎（AD）患者数量一直在增加。据报道，三丁基锡（TBT）化合物不仅对生殖系统有影响，对免疫系统也有影响。
为了研究TBT是否对AD有影响，我们给在常规条件下会自发发生皮炎的DS-Nh小鼠喂食含TBT的饮食。
对喂食TBT或对照饮食的DS-Nh小鼠进行皮肤变化、皮肤上金黄色葡萄球菌数量和血清IgE水平的检查。为了确定淋巴细胞产生的Th1/Th2细胞因子，将喂食TBT的DS-Nh小鼠和对照小鼠的淋巴细胞与葡萄球菌肠毒素B一起培养，并用ELISA测量上清液中的细胞因子水平。我们不仅观察了自发皮炎，还观察了用2,4,6-三硝基氯苯（TNCB）致敏诱导的皮炎。
TNCB致敏诱导的AD样病变在喂食TBT的小鼠中比在喂食对照饮食的小鼠中更严重。在喂食TBT的小鼠中观察到皮肤上金黄色葡萄球菌的增加比喂食对照饮食的小鼠更大。在喂食TBT饮食并用TNCB处理的小鼠中观察到IFN-γ产生减少，IL-5和IL-13产生增加。这些发现表明，TBT诱导的金黄色葡萄球菌增加和Th2反应增强加剧了用TNCB处理的小鼠中的AD样病变。
