Takahashi Hinako, Nakazawa Masatoshi, Takahashi Kazuo, Aihara Michiko, Minami Mutsuhiko, Hirasawa Tsutomu, Ikezawa Zenro
Department of Environmental Immuno-Dermatology, Yokohama City University Graduate School of Medicine, Kanagawa, Japan.
J Dermatol Sci. 2008 Apr;50(1):31-9. doi: 10.1016/j.jdermsci.2007.11.002. Epub 2008 Jan 8.
Zinc is one of the essential dietary factors and zinc deficiency diminishes the immune system. However, the mechanisms by which zinc deficiency affects the immune system are not fully understood.
We analyzed the mechanisms of zinc deficiency affecting the allergic response using a DS-Nh mouse model of atopic dermatitis.
Male DS-Nh mice were fed a zinc deficient diet for 4 weeks. We measured transepidermal water loss (TEWL) and epidermal moisture level, assessed the skin eruption score, and examined the frequency of lymphocyte subpopulation in spleen and thymus by flow cytometry. The suppressive effect of CD25+CD4+ T cells was analyzed in vitro. The amount of cytokines produced by the spleen cells and the serum IgE levels were measured by ELISA.
In DS-Nh mice fed the zinc deficient diet, skin eruptions were exacerbated and serum IgE levels and number of S. aureus on the skin surface was increased. IFN-gamma and IL-13 production by spleen cells was increased. The number of CD25+CD4+ T cells in spleen was significantly decreased, while the percentage of Foxp3 positive cells in the CD25+CD4+ T cells was comparable to those of the controls. CD25+CD4+ T cells from mice fed the zinc deficient diet maintained a suppressive function compared with those from the controls.
These findings indicate that zinc deficiency influences the skin barrier system and immune system, and suggests that zinc deficiency acts as an exacerbation factor of atopic dermatitis.
锌是必需的膳食因素之一，锌缺乏会削弱免疫系统。然而，锌缺乏影响免疫系统的机制尚未完全明确。
我们使用特应性皮炎的DS-Nh小鼠模型分析锌缺乏影响过敏反应的机制。
给雄性DS-Nh小鼠喂食缺锌饮食4周。我们测量经表皮水分流失（TEWL）和表皮水分水平，评估皮肤疹评分，并通过流式细胞术检测脾脏和胸腺中淋巴细胞亚群的频率。在体外分析CD25+CD4+T细胞的抑制作用。通过酶联免疫吸附测定法（ELISA）测量脾细胞产生的细胞因子量和血清IgE水平。
在喂食缺锌饮食的DS-Nh小鼠中，皮肤疹加剧，皮肤表面的血清IgE水平和金黄色葡萄球菌数量增加。脾细胞产生的干扰素-γ（IFN-γ）和白细胞介素-13（IL-13）增加。脾脏中CD25+CD4+T细胞的数量显著减少，而CD25+CD4+T细胞中叉头框蛋白3（Foxp3）阳性细胞的百分比与对照组相当。与对照组相比，喂食缺锌饮食的小鼠的CD25+CD4+T细胞保持抑制功能。
这些发现表明锌缺乏影响皮肤屏障系统和免疫系统，并提示锌缺乏是特应性皮炎的加重因素。
