Igyártó Botond-Zoltán, Magyar Attila, Oláh Imre
Department of Human Morphology and Developmental Biology, Faculty of Medicine, Semmelweis University, H-1094 Tuzoltó u. 58., Budapest, Hungary.
Cell Tissue Res. 2007 Jan;327(1):83-92. doi: 10.1007/s00441-006-0250-0. Epub 2006 Aug 29.
The ellipsoid-associated cell (EAC) is a blood-borne phagocytic cell, residing in the antigen trapping zone of the chicken spleen. Binding and endocytosis of betaGalactosidase (betaGal) are independent from the Fc and complement receptors, because sulfated polysaccharides, in a concentration manner, inhibit the bacterial antigen uptake. The betaGal-positive cells migrate to the periarterial lymphatic sheath (PALS), the preexisting germinal centers (GC), and form clusters with B- and T-cells. betaGal, E5G12 double positive cells on the surface of the ellipsoid and in the PALS, GC and clusters prove that the EACs carry the enzyme. The EAC and the follicular dendritic cell (FDC) express, 68.2 and E5G12 and, 74.3 and E5G12, antigens, respectively. During migration the cessation of 68.2 and expression of 74.3 indicate the differentiation of EAC to FDC. By day 14 the clusters had disappeared, and in several GC the presence of double positive cells (74.3 and betaGal; E5G12 and betaGal) showed that the clusters had developed to GC. The presence of betaGal(+) cells in the PALS, where interdigitating dendritic cells (IDC) cooperate with the T-cells, suggests that in the spleen alternate routes exist for the EAC differentiation to FDC: EAC to FDC: betaGal-loaded cells in the preexisting GC; and EAC through IDC to FDC: betaGal(+) EAC in the PALS and clusters. The EAC-FDC axis works exclusively inside the spleen; therefore; this system may be operated in pneumococcus infection.
椭球体相关细胞（EAC）是一种血源性吞噬细胞，存在于鸡脾脏的抗原捕获区。β-半乳糖苷酶（βGal）的结合和内吞作用独立于Fc和补体受体，因为硫酸化多糖以浓度依赖的方式抑制细菌抗原的摄取。βGal阳性细胞迁移至动脉周围淋巴鞘（PALS）、已有的生发中心（GC），并与B细胞和T细胞形成簇。椭球体表面以及PALS、GC和簇中的βGal、E5G12双阳性细胞证明EAC携带该酶。EAC和滤泡树突状细胞（FDC）分别表达68.2和E5G12以及74.3和E5G12抗原。在迁移过程中，68.2表达的停止和74.3的表达表明EAC向FDC分化。到第14天，簇消失，在几个GC中双阳性细胞（74.3和βGal；E5G12和βGal）的存在表明簇已发育为GC。在PALS中存在βGal(+)细胞，此处交错树突状细胞（IDC）与T细胞协作，这表明在脾脏中存在EAC向FDC分化的替代途径：EAC向FDC：已有的GC中加载βGal的细胞；以及EAC通过IDC向FDC：PALS和簇中的βGal(+) EAC。EAC - FDC轴仅在脾脏内部起作用；因此；该系统可能在肺炎球菌感染中发挥作用。
