del Cacho E, Gallego M, Arnal C, Bascuas J A
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Spain.
Anat Rec. 1995 Jan;241(1):105-12. doi: 10.1002/ar.1092410114.
The objective of the present study is to investigate the migration pattern of the splenic dendritic cell of the chicken named the ellipsoid-associated cell (EAC) from the site of initial location at the periphery of the ellipsoid to the splenic T- and B-dependent areas.
Bovine serum albumin bound to biotin and conjugated to gold particles was used as a histochemically identifiable antigen detected as a peroxidase reaction. The antigen was intravenously injected, and subsequently its pattern of distribution in a time sequence and within the tissue was examined at the light and electron microscopy levels. In addition, an hour prior to sacrifice, the chickens received a single injection of the thymidine analogue 5-bromo-2'-deoxyuridine, in order to quantify the number of DNA synthesizing cells and to establish a relationship between the migrating EAC and the rate of mitosis in the white pulp.
The observations showed that between 12 hours and 3 days after the second antigen administration the labeled EAC, which was first located around the ellipsoid, progressively reached further areas with time towards the periarteriolar lymphoid sheaths, where newly formed germinal centers appeared. Furthermore, the rate of cell proliferation within the white pulp was associated with the arrival of the antigen-transporting EAC.
The results suggest that migrating EAC have a role as both antigen-transporting cell and antigen-presenting cell in the T- and B-dependent areas, as a result of which migrating EAC is transiently found in periellipsoidal white pulp, then periarteriolar lymphoid sheaths, and finally germinal centers, where it may function as an interdigitating cell or as a follicular dendritic cell, depending on its location. Thus, we conclude that the EACs are precursors of both interdigitating and follicular dendritic cells.
本研究的目的是调查鸡的脾树突状细胞——椭球体相关细胞（EAC）从椭球体周边的初始位置迁移至脾T细胞依赖区和B细胞依赖区的模式。
将与生物素结合并偶联金颗粒的牛血清白蛋白用作通过过氧化物酶反应检测的组织化学可识别抗原。静脉注射该抗原，随后在光学显微镜和电子显微镜水平上按时间顺序检查其在组织内的分布模式。此外，在处死前一小时，给鸡单次注射胸苷类似物5-溴-2'-脱氧尿苷，以量化DNA合成细胞的数量，并确定迁移的EAC与白髓有丝分裂率之间的关系。
观察结果显示，在第二次给予抗原后的12小时至3天之间，最初位于椭球体周围的标记EAC随着时间的推移逐渐到达更远的区域，朝向动脉周围淋巴鞘，在那里出现了新形成的生发中心。此外，白髓内的细胞增殖率与抗原转运EAC的到达有关。
结果表明，迁移的EAC在T细胞依赖区和B细胞依赖区中既作为抗原转运细胞又作为抗原呈递细胞发挥作用，因此，迁移的EAC首先短暂出现在椭球体周围白髓中，然后出现在动脉周围淋巴鞘中，最后出现在生发中心，在那里它可能根据其位置充当交错突细胞或滤泡树突状细胞。因此，我们得出结论，EAC是交错突细胞和滤泡树突状细胞的前体。
