Lankiewicz Silke, Rivero Bertha Gutierrez, Bócher Oliver
AdnaGen AG, Ostpassage 7, 30583 Langenhagen, Germany.
Mol Biotechnol. 2006 Sep;34(1):15-27. doi: 10.1385/MB:34:1:15.
Detection of disseminated tumor cells in the blood circulation is important in assessing tumor progression. The objective of this examination was to develop a highly specific and sensitive quantitative realtime reverse transcriptase-polymerase chain reaction (qRT-PCR) assay for the detection of relevant tumor-associated transcripts in patients' blood. The qRT-PCR assays detect the human epidermal growth factor receptor 2 (HER2) and CK20 transcripts of two tumor cells spiked into 5 mL of blood after an immunomagnetic tumor cell enrichment. Furthermore, the HER2 assay is only specific when enrichment is included. This procedure is a useful alternative to fluorescence in situ hybridization and immunocytochemistry for gene alteration analysis in human tumors. The analysis of the studied molecular markers of tumor cells in blood may be useful in the detection of disseminated tumor cells as well as for monitoring treatment response, early detection of relapse, and for stratification of patients with carcinoma.
检测血液循环中的播散肿瘤细胞对于评估肿瘤进展具有重要意义。本检查的目的是开发一种高度特异且灵敏的定量实时逆转录聚合酶链反应（qRT-PCR）检测方法，用于检测患者血液中相关的肿瘤相关转录本。qRT-PCR检测方法在免疫磁珠富集肿瘤细胞后，可检测添加到5 mL血液中的两种肿瘤细胞的人表皮生长因子受体2（HER2）和细胞角蛋白20（CK20）转录本。此外，HER2检测仅在包含富集步骤时才具有特异性。该方法是用于人类肿瘤基因改变分析的荧光原位杂交和免疫细胞化学的一种有用替代方法。分析血液中肿瘤细胞的研究分子标志物可能有助于检测播散肿瘤细胞，以及监测治疗反应、早期复发检测和癌症患者分层。
