Zieglschmid Veit, Hollmann Christiane, Gutierrez Bertha, Albert Winfried, Strothoff Dark, Gross Eberhard, Böcher Oliver
AdnaGen AG, Ostpassage 7, D-30853 Langenhagen, Germany.
Anticancer Res. 2005 May-Jun;25(3A):1803-10.
A highly specific and sensitive tumor cell detection assay is reported, which combines immunomagnetic enrichment with multiplex RT-PCR analysis.
The effect on the recovery rate of breast, testicular and colorectal cancer cells using single antibodies and combinations of them for IMS was examined by fluorescence microscopy and multiplex RT-PCR. The clinical utility of a tumor cell detection assay using IMS with multiplex RT-PCR was tested by examination of colorectal cancer blood samples and by comparing the results with CEA serum protein levels.
A combination of antibodies for IMS and multiplex RT-PCR analysis proved to be the most sensitive approach for detection of tumor cells in peripheral blood with a detection limit of two tumor cells. The examination of blood of colorectal cancer patients by using a multiplex RT-PCR assay in comparison with CEA serum protein levels indicated a distinct advantage of the former over the latter with respect to a more reliable prediction of an ongoing metastatic process.
The results indicate that a combination of antibodies for immunomagnetic enrichment with multiplex RT-PCR analysis detects disseminated tumor cells with high sensitivity and specificity, thus indicating a metastatic process several months earlier compared to CEA serum protein level measurements. This assay might be valuable for prognosis in cancer.
报道了一种高度特异性和敏感性的肿瘤细胞检测方法，该方法将免疫磁珠富集与多重逆转录聚合酶链反应（RT-PCR）分析相结合。
通过荧光显微镜和多重RT-PCR检测单克隆抗体及其组合用于免疫磁珠分选（IMS）时对乳腺癌、睾丸癌和结肠癌细胞回收率的影响。通过检测结肠癌血样并将结果与癌胚抗原（CEA）血清蛋白水平进行比较，来测试使用免疫磁珠分选结合多重RT-PCR的肿瘤细胞检测方法的临床实用性。
免疫磁珠分选抗体与多重RT-PCR分析相结合被证明是检测外周血中肿瘤细胞最敏感的方法，检测限为两个肿瘤细胞。与CEA血清蛋白水平相比，使用多重RT-PCR检测结肠癌患者血液表明，前者在更可靠地预测正在进行的转移过程方面明显优于后者。
结果表明，免疫磁珠富集抗体与多重RT-PCR分析相结合能够高灵敏度和特异性地检测播散性肿瘤细胞，因此与CEA血清蛋白水平检测相比，能提前数月指示转移过程。该检测方法可能对癌症预后评估有价值。
