Ballester Maria, Sánchez Armand, Santaló Josep, Folch Josep M, Ibáñez Elena
Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain.
Mol Biotechnol. 2006 Sep;34(1):37-44. doi: 10.1385/MB:34:1:37.
In this article, we describe the use of a caprine beta-lactoglobulin (betaLG) expression cassette previously obtained by our group to target the expression of the human follicle-stimulating hormone (hFSH) to the mammary gland of transgenic mice. The hFSH is a pituitary glycoprotein composed of two subunits (alpha and beta). At present, this protein is obtained from mammalian cellular fermentors, and it is extensively used in the treatment of human infertility. Four lines of double (hFSHalpha/beta) transgenic mice that stably transmitted the transgenes were obtained, and hFSHalpha and hFSHbeta mRNA was detected by reverse transcriptase-polymerase chain reaction in the mammary gland of lactating females from all four transgenic lines. The hFSH protein was present in the mammary gland of the lactating females, but could not be detected in the milk by Western blot, probably as a result of low levels of transgene expression.
在本文中，我们描述了利用我们小组先前获得的一个山羊β-乳球蛋白（βLG）表达盒，将人促卵泡激素（hFSH）的表达靶向到转基因小鼠的乳腺。hFSH是一种由两个亚基（α和β）组成的垂体糖蛋白。目前，这种蛋白质是从哺乳动物细胞发酵罐中获得的，并且广泛用于治疗人类不孕症。获得了四条稳定传递转基因的双（hFSHα/β）转基因小鼠品系，通过逆转录-聚合酶链反应在所有四个转基因品系的泌乳雌性小鼠的乳腺中检测到了hFSHα和hFSHβ mRNA。hFSH蛋白存在于泌乳雌性小鼠的乳腺中，但通过蛋白质免疫印迹法在乳汁中未检测到，这可能是由于转基因表达水平较低所致。
