抗肠道病毒70型的中和单克隆抗体与病毒蛋白1C和1D发生反应。

Neutralizing monoclonal antibody against enterovirus-70 reacts with viral proteins 1C and 1D.

作者信息

Wiley J A, Brodeur B R, Dimock K D, Sattar S A

机构信息

National Laboratory for Immunology, Laboratory Center for Disease Control, Ottawa, Ontario, Canada.

出版信息

Viral Immunol. 1990 Summer;3(2):137-46. doi: 10.1089/vim.1990.3.137.

DOI:10.1089/vim.1990.3.137

PMID:1694428

Abstract

A library of murine monoclonal antibodies against the prototype Enterovirus-70 (EV-70) strain, J670/71, was made for the purpose of studying the immunologically reactive determinants of the virus. Each of the monoclonal antibodies reacted with several other strains of Enterovirus-70 when tested by immunofluorescence. However, none of these monoclonal antibodies reacted with any other picornavirus tested. It was found that all of the monoclonal antibodies precipitated EV-70 viral proteins 1C and 1D in radio-immunoprecipitation assays. However, only one of these monoclonal antibodies, an IgG3 kappa, was capable of neutralizing the virus.

摘要

为了研究肠道病毒70型（EV - 70）原型株J670/71的免疫反应性决定簇，构建了一个针对该病毒的鼠单克隆抗体文库。通过免疫荧光检测时，每种单克隆抗体都能与其他几种肠道病毒70型毒株发生反应。然而，这些单克隆抗体均未与所检测的任何其他小RNA病毒发生反应。研究发现，在放射免疫沉淀试验中，所有这些单克隆抗体都能沉淀EV - 70病毒蛋白1C和1D。不过，这些单克隆抗体中只有一种IgG3 κ能够中和该病毒。