Yousef G E, Brown I N, Mowbray J F
Department of Experimental Pathology, St. Mary's Hospital Medical School, London, UK.
Intervirology. 1987;28(3):163-70. doi: 10.1159/000150012.
This paper describes the derivation and characterization of monoclonal antibodies reactive with the group-specific epitopes of the VP1 peptide shared by enteroviruses. When tested against a wide range of the prototype strains of Coxsackie viruses A and B, polioviruses, and echoviruses, the monoclonal antibodies reacted with all of them in two or more assay systems. The virus of hepatitis A was the only enterovirus tested which was not recognized by these monoclonal antibodies. This finding is consistent with recent reports demonstrating genomic dissimilarity between this virus and the other enteroviruses. Although monoclonal antibodies have frequently been found to be assay specific, these particular antibodies were found to be equally effective in different immunochemical and biological assay systems. Biochemical characterization of one of these monoclonal antibodies is described and its potential usefulness for diagnosis is discussed.
本文描述了与肠道病毒共有的VP1肽的群特异性表位发生反应的单克隆抗体的衍生和特性。当针对广泛的柯萨奇病毒A和B、脊髓灰质炎病毒及埃可病毒的原型毒株进行检测时，这些单克隆抗体在两种或更多检测系统中均与它们发生反应。甲型肝炎病毒是所检测的唯一未被这些单克隆抗体识别的肠道病毒。这一发现与最近表明该病毒与其他肠道病毒在基因组上存在差异的报告一致。尽管经常发现单克隆抗体具有检测特异性，但这些特定抗体在不同的免疫化学和生物学检测系统中同样有效。本文描述了其中一种单克隆抗体的生化特性，并讨论了其在诊断方面的潜在用途。
